Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26170 and PS417_26175 are separated by 10 nucleotides PS417_26175 and PS417_26180 are separated by 123 nucleotides PS417_26180 and PS417_26185 overlap by 4 nucleotides PS417_26185 and PS417_26190 are separated by 15 nucleotides
PS417_26170: PS417_26170 - formyltetrahydrofolate deformylase, at 5,654,431 to 5,655,288
_26170
PS417_26175: PS417_26175 - sarcosine oxidase subunit gamma, at 5,655,299 to 5,655,931
_26175
PS417_26180: PS417_26180 - sarcosine oxidase subunit alpha, at 5,656,055 to 5,659,072
_26180
PS417_26185: PS417_26185 - sarcosine oxidase subunit delta, at 5,659,069 to 5,659,371
_26185
PS417_26190: PS417_26190 - sarcosine oxidase subunit beta, at 5,659,387 to 5,660,637
_26190
Position (kb) 5656
5657
5658
5659
5660 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5655.184 kb on - strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.293 kb on + strand at 5655.486 kb on + strand, within PS417_26175 at 5655.486 kb on + strand, within PS417_26175 at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.932 kb on - strand at 5656.342 kb on + strand at 5656.409 kb on - strand, within PS417_26180 at 5656.502 kb on + strand, within PS417_26180 at 5656.503 kb on - strand, within PS417_26180 at 5656.571 kb on + strand, within PS417_26180 at 5656.585 kb on - strand, within PS417_26180 at 5656.640 kb on + strand, within PS417_26180 at 5656.671 kb on - strand, within PS417_26180 at 5656.797 kb on + strand, within PS417_26180 at 5656.798 kb on - strand, within PS417_26180 at 5656.830 kb on + strand at 5657.002 kb on - strand, within PS417_26180 at 5657.002 kb on - strand, within PS417_26180 at 5657.148 kb on + strand, within PS417_26180 at 5657.149 kb on - strand, within PS417_26180 at 5657.544 kb on + strand, within PS417_26180 at 5657.544 kb on + strand, within PS417_26180 at 5658.126 kb on + strand, within PS417_26180 at 5658.127 kb on - strand, within PS417_26180 at 5658.583 kb on - strand, within PS417_26180 at 5658.639 kb on + strand, within PS417_26180 at 5658.640 kb on - strand, within PS417_26180 at 5658.690 kb on + strand, within PS417_26180 at 5658.690 kb on + strand, within PS417_26180 at 5658.795 kb on + strand at 5658.796 kb on - strand at 5659.025 kb on - strand at 5659.124 kb on + strand, within PS417_26185 at 5659.124 kb on + strand, within PS417_26185 at 5659.143 kb on - strand, within PS417_26185 at 5659.172 kb on + strand, within PS417_26185 at 5659.172 kb on + strand, within PS417_26185 at 5659.172 kb on + strand, within PS417_26185 at 5659.244 kb on + strand, within PS417_26185 at 5659.245 kb on - strand, within PS417_26185 at 5659.245 kb on - strand, within PS417_26185 at 5659.362 kb on + strand at 5659.362 kb on + strand at 5659.363 kb on - strand at 5659.371 kb on + strand at 5659.737 kb on - strand, within PS417_26190 at 5659.790 kb on + strand, within PS417_26190 at 5659.791 kb on - strand, within PS417_26190
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 5,655,184 - PS417_26170 0.88 -0.6 5,655,188 + PS417_26170 0.88 +1.6 5,655,188 + PS417_26170 0.88 +2.8 5,655,189 - PS417_26170 0.88 +0.8 5,655,189 - PS417_26170 0.88 +0.1 5,655,189 - PS417_26170 0.88 -0.3 5,655,293 + +1.7 5,655,486 + PS417_26175 0.30 +1.6 5,655,486 + PS417_26175 0.30 -1.4 5,655,914 + -0.1 5,655,914 + -0.2 5,655,914 + -0.1 5,655,914 + -0.9 5,655,914 + -0.3 5,655,915 - +0.4 5,655,915 - +0.8 5,655,915 - +0.2 5,655,932 - -0.2 5,656,342 + -2.4 5,656,409 - PS417_26180 0.12 -1.0 5,656,502 + PS417_26180 0.15 -1.2 5,656,503 - PS417_26180 0.15 -1.2 5,656,571 + PS417_26180 0.17 +0.8 5,656,585 - PS417_26180 0.18 -1.0 5,656,640 + PS417_26180 0.19 -0.9 5,656,671 - PS417_26180 0.20 -1.0 5,656,797 + PS417_26180 0.25 -1.0 5,656,798 - PS417_26180 0.25 -1.0 5,656,830 + +0.2 5,657,002 - PS417_26180 0.31 -0.8 5,657,002 - PS417_26180 0.31 -0.9 5,657,148 + PS417_26180 0.36 +0.6 5,657,149 - PS417_26180 0.36 +0.1 5,657,544 + PS417_26180 0.49 -0.8 5,657,544 + PS417_26180 0.49 -0.0 5,658,126 + PS417_26180 0.69 +0.0 5,658,127 - PS417_26180 0.69 -1.0 5,658,583 - PS417_26180 0.84 +0.5 5,658,639 + PS417_26180 0.86 +1.8 5,658,640 - PS417_26180 0.86 -0.5 5,658,690 + PS417_26180 0.87 +0.1 5,658,690 + PS417_26180 0.87 -1.0 5,658,795 + +0.2 5,658,796 - +1.2 5,659,025 - -1.5 5,659,124 + PS417_26185 0.18 -0.1 5,659,124 + PS417_26185 0.18 -0.9 5,659,143 - PS417_26185 0.24 +1.3 5,659,172 + PS417_26185 0.34 +1.0 5,659,172 + PS417_26185 0.34 +0.4 5,659,172 + PS417_26185 0.34 +0.2 5,659,244 + PS417_26185 0.58 -1.5 5,659,245 - PS417_26185 0.58 -0.2 5,659,245 - PS417_26185 0.58 -0.7 5,659,362 + -1.8 5,659,362 + +1.7 5,659,363 - -0.9 5,659,371 + +0.8 5,659,737 - PS417_26190 0.28 -1.3 5,659,790 + PS417_26190 0.32 -1.8 5,659,791 - PS417_26190 0.32 +3.4
Or see this region's nucleotide sequence