Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23605 and PS417_23610 are separated by 194 nucleotides PS417_23610 and PS417_23615 are separated by 122 nucleotides
PS417_23605: PS417_23605 - permease DsdX, at 5,104,968 to 5,106,371
_23605
PS417_23610: PS417_23610 - hypothetical protein, at 5,106,566 to 5,106,946
_23610
PS417_23615: PS417_23615 - sulfate transporter, at 5,107,069 to 5,108,514
_23615
Position (kb)
5106
5107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5105.622 kb on - strand, within PS417_23605 at 5105.768 kb on - strand, within PS417_23605 at 5105.768 kb on - strand, within PS417_23605 at 5105.768 kb on - strand, within PS417_23605 at 5105.797 kb on + strand, within PS417_23605 at 5105.798 kb on - strand, within PS417_23605 at 5105.798 kb on - strand, within PS417_23605 at 5106.283 kb on + strand at 5106.284 kb on - strand at 5106.284 kb on - strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.481 kb on - strand at 5106.481 kb on - strand at 5106.481 kb on - strand at 5106.530 kb on - strand at 5106.530 kb on - strand at 5106.595 kb on + strand at 5106.635 kb on - strand, within PS417_23610 at 5106.740 kb on + strand, within PS417_23610 at 5106.740 kb on + strand, within PS417_23610 at 5106.740 kb on + strand at 5106.811 kb on - strand, within PS417_23610 at 5106.815 kb on + strand at 5106.815 kb on + strand, within PS417_23610 at 5106.962 kb on + strand at 5107.056 kb on + strand at 5107.847 kb on + strand, within PS417_23615
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 5,105,622 - PS417_23605 0.47 +1.1 5,105,768 - PS417_23605 0.57 +1.7 5,105,768 - PS417_23605 0.57 -0.8 5,105,768 - PS417_23605 0.57 -2.1 5,105,797 + PS417_23605 0.59 -1.3 5,105,798 - PS417_23605 0.59 -2.4 5,105,798 - PS417_23605 0.59 +1.0 5,106,283 + -2.3 5,106,284 - -0.3 5,106,284 - -1.7 5,106,480 + +0.1 5,106,480 + +0.3 5,106,480 + -0.3 5,106,480 + -0.3 5,106,480 + +0.7 5,106,481 - +0.7 5,106,481 - +1.7 5,106,481 - +3.7 5,106,530 - +0.5 5,106,530 - -1.0 5,106,595 + +0.5 5,106,635 - PS417_23610 0.18 -0.7 5,106,740 + PS417_23610 0.46 -1.6 5,106,740 + PS417_23610 0.46 +0.0 5,106,740 + -0.9 5,106,811 - PS417_23610 0.64 -1.6 5,106,815 + -0.9 5,106,815 + PS417_23610 0.65 -3.0 5,106,962 + +1.4 5,107,056 + -1.1 5,107,847 + PS417_23615 0.54 +0.5
Or see this region's nucleotide sequence