Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02050 and PS417_02055 overlap by 4 nucleotides PS417_02055 and PS417_02060 overlap by 4 nucleotides PS417_02060 and PS417_02065 are separated by 33 nucleotides
PS417_02050: PS417_02050 - hypothetical protein, at 459,972 to 460,454
_02050
PS417_02055: PS417_02055 - methyltransferase, at 460,451 to 461,185
_02055
PS417_02060: PS417_02060 - FAD-dependent oxidoreductase, at 461,182 to 462,429
_02060
PS417_02065: PS417_02065 - membrane protein, at 462,463 to 464,775
_02065
Position (kb) 461
462
463 Strain fitness (log2 ratio) -2
-1
0
1 at 460.716 kb on + strand, within PS417_02055 at 460.716 kb on + strand, within PS417_02055 at 460.717 kb on - strand, within PS417_02055 at 460.717 kb on - strand, within PS417_02055 at 460.746 kb on + strand, within PS417_02055 at 460.747 kb on - strand, within PS417_02055 at 461.059 kb on + strand, within PS417_02055 at 461.068 kb on + strand, within PS417_02055 at 461.459 kb on - strand, within PS417_02060 at 461.814 kb on + strand, within PS417_02060 at 461.815 kb on - strand, within PS417_02060 at 461.992 kb on + strand, within PS417_02060 at 462.226 kb on + strand, within PS417_02060 at 462.328 kb on + strand at 462.328 kb on + strand at 462.328 kb on + strand at 462.328 kb on + strand at 462.329 kb on - strand at 462.329 kb on - strand at 462.329 kb on - strand at 462.421 kb on + strand at 462.421 kb on + strand at 462.466 kb on + strand at 462.467 kb on - strand at 462.467 kb on - strand at 462.504 kb on + strand at 462.562 kb on - strand at 462.625 kb on + strand at 463.063 kb on + strand, within PS417_02065 at 463.063 kb on + strand, within PS417_02065 at 463.090 kb on - strand, within PS417_02065
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 460,716 + PS417_02055 0.36 -1.0 460,716 + PS417_02055 0.36 -1.5 460,717 - PS417_02055 0.36 -0.7 460,717 - PS417_02055 0.36 +1.5 460,746 + PS417_02055 0.40 -1.7 460,747 - PS417_02055 0.40 -0.9 461,059 + PS417_02055 0.83 +1.4 461,068 + PS417_02055 0.84 -0.2 461,459 - PS417_02060 0.22 -0.3 461,814 + PS417_02060 0.51 -2.5 461,815 - PS417_02060 0.51 -0.6 461,992 + PS417_02060 0.65 -0.2 462,226 + PS417_02060 0.84 -1.5 462,328 + +0.5 462,328 + +1.1 462,328 + -0.0 462,328 + -0.1 462,329 - +1.1 462,329 - +0.7 462,329 - -2.0 462,421 + -0.0 462,421 + -2.0 462,466 + +1.0 462,467 - +1.6 462,467 - +1.1 462,504 + +0.3 462,562 - +0.4 462,625 + -1.0 463,063 + PS417_02065 0.26 +0.1 463,063 + PS417_02065 0.26 -1.8 463,090 - PS417_02065 0.27 -1.2
Or see this region's nucleotide sequence