Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01945 and PS417_01950 are separated by 181 nucleotides PS417_01950 and PS417_01955 are separated by 191 nucleotides
PS417_01945: PS417_01945 - nuclease, at 431,076 to 431,870
_01945
PS417_01950: PS417_01950 - malate dehydrogenase, at 432,052 to 433,320
_01950
PS417_01955: PS417_01955 - peptidase, at 433,512 to 435,974
_01955
Position (kb) 432
433
434 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 431.302 kb on - strand, within PS417_01945 at 431.419 kb on - strand, within PS417_01945 at 431.508 kb on - strand, within PS417_01945 at 431.922 kb on - strand at 431.935 kb on - strand at 431.955 kb on - strand at 432.007 kb on + strand at 432.035 kb on - strand at 432.051 kb on + strand at 432.051 kb on + strand at 432.116 kb on + strand at 432.117 kb on - strand at 432.117 kb on - strand at 432.163 kb on + strand at 432.164 kb on - strand at 432.227 kb on - strand, within PS417_01950 at 432.227 kb on - strand, within PS417_01950 at 432.327 kb on + strand, within PS417_01950 at 432.328 kb on - strand, within PS417_01950 at 432.697 kb on + strand, within PS417_01950 at 432.698 kb on - strand at 432.698 kb on - strand, within PS417_01950 at 432.751 kb on + strand, within PS417_01950 at 433.174 kb on + strand, within PS417_01950 at 433.175 kb on - strand, within PS417_01950 at 433.318 kb on + strand at 433.385 kb on - strand at 433.513 kb on + strand at 433.514 kb on - strand at 433.627 kb on + strand at 433.745 kb on + strand at 433.748 kb on - strand at 433.748 kb on - strand at 433.816 kb on + strand, within PS417_01955 at 433.852 kb on + strand, within PS417_01955 at 433.984 kb on + strand, within PS417_01955 at 433.984 kb on + strand, within PS417_01955 at 433.985 kb on - strand, within PS417_01955 at 433.985 kb on - strand, within PS417_01955 at 433.985 kb on - strand, within PS417_01955 at 434.233 kb on + strand, within PS417_01955
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 431,302 - PS417_01945 0.28 -0.6 431,419 - PS417_01945 0.43 +1.1 431,508 - PS417_01945 0.54 -0.6 431,922 - -2.0 431,935 - +0.7 431,955 - -1.7 432,007 + -0.7 432,035 - -0.4 432,051 + +0.6 432,051 + +2.4 432,116 + +1.5 432,117 - +1.2 432,117 - +2.1 432,163 + +0.5 432,164 - +0.1 432,227 - PS417_01950 0.14 +0.4 432,227 - PS417_01950 0.14 -0.2 432,327 + PS417_01950 0.22 +1.5 432,328 - PS417_01950 0.22 +2.1 432,697 + PS417_01950 0.51 -2.7 432,698 - +1.8 432,698 - PS417_01950 0.51 +0.4 432,751 + PS417_01950 0.55 +0.7 433,174 + PS417_01950 0.88 +0.8 433,175 - PS417_01950 0.88 -1.3 433,318 + -2.3 433,385 - -1.7 433,513 + -0.3 433,514 - -0.2 433,627 + -1.0 433,745 + +2.5 433,748 - +1.8 433,748 - +1.8 433,816 + PS417_01955 0.12 +1.5 433,852 + PS417_01955 0.14 +3.2 433,984 + PS417_01955 0.19 +2.8 433,984 + PS417_01955 0.19 +2.6 433,985 - PS417_01955 0.19 +1.6 433,985 - PS417_01955 0.19 +2.3 433,985 - PS417_01955 0.19 +2.5 434,233 + PS417_01955 0.29 +2.8
Or see this region's nucleotide sequence