Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05475 and PS417_05480 overlap by 4 nucleotides PS417_05480 and PS417_05485 overlap by 4 nucleotides PS417_05485 and PS417_05490 are separated by 80 nucleotides PS417_05490 and PS417_05495 are separated by 55 nucleotides
PS417_05475: PS417_05475 - arginase, at 1,233,548 to 1,234,468
_05475
PS417_05480: PS417_05480 - transporter, at 1,234,465 to 1,234,845
_05480
PS417_05485: PS417_05485 - transporter, at 1,234,842 to 1,235,246
_05485
PS417_05490: PS417_05490 - fatty acid desaturase, at 1,235,327 to 1,236,199
_05490
PS417_05495: PS417_05495 - structural protein MipA, at 1,236,255 to 1,237,025
_05495
Position (kb)
1235
1236
1237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1235.285 kb on + strand at 1235.285 kb on + strand at 1235.285 kb on + strand at 1235.511 kb on + strand, within PS417_05490 at 1235.512 kb on - strand, within PS417_05490 at 1235.512 kb on - strand, within PS417_05490 at 1235.524 kb on - strand, within PS417_05490 at 1235.577 kb on + strand, within PS417_05490 at 1235.577 kb on + strand at 1235.578 kb on - strand, within PS417_05490 at 1235.578 kb on - strand, within PS417_05490 at 1235.580 kb on + strand, within PS417_05490 at 1235.653 kb on - strand, within PS417_05490 at 1235.670 kb on + strand, within PS417_05490 at 1235.670 kb on + strand, within PS417_05490 at 1235.671 kb on - strand, within PS417_05490 at 1235.671 kb on - strand, within PS417_05490 at 1235.671 kb on - strand, within PS417_05490 at 1235.745 kb on + strand, within PS417_05490 at 1235.787 kb on + strand, within PS417_05490 at 1235.788 kb on - strand, within PS417_05490 at 1235.788 kb on - strand, within PS417_05490 at 1235.988 kb on + strand, within PS417_05490 at 1236.266 kb on + strand at 1236.269 kb on - strand at 1236.653 kb on - strand, within PS417_05495 at 1236.653 kb on - strand, within PS417_05495 at 1236.653 kb on - strand, within PS417_05495 at 1236.653 kb on - strand, within PS417_05495 at 1236.679 kb on + strand, within PS417_05495 at 1236.763 kb on + strand, within PS417_05495 at 1236.764 kb on - strand, within PS417_05495 at 1236.770 kb on + strand, within PS417_05495 at 1236.771 kb on - strand, within PS417_05495 at 1236.773 kb on - strand, within PS417_05495 at 1236.773 kb on - strand, within PS417_05495 at 1236.866 kb on - strand, within PS417_05495 at 1237.047 kb on + strand at 1237.047 kb on + strand at 1237.047 kb on + strand at 1237.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 1,235,285 + -0.7 1,235,285 + -0.5 1,235,285 + -1.1 1,235,511 + PS417_05490 0.21 -0.3 1,235,512 - PS417_05490 0.21 +0.8 1,235,512 - PS417_05490 0.21 +1.6 1,235,524 - PS417_05490 0.23 +0.2 1,235,577 + PS417_05490 0.29 -0.1 1,235,577 + +0.7 1,235,578 - PS417_05490 0.29 -2.3 1,235,578 - PS417_05490 0.29 -0.9 1,235,580 + PS417_05490 0.29 +0.2 1,235,653 - PS417_05490 0.37 +2.9 1,235,670 + PS417_05490 0.39 -1.1 1,235,670 + PS417_05490 0.39 -0.2 1,235,671 - PS417_05490 0.39 +0.7 1,235,671 - PS417_05490 0.39 +0.0 1,235,671 - PS417_05490 0.39 +1.4 1,235,745 + PS417_05490 0.48 +0.1 1,235,787 + PS417_05490 0.53 +1.9 1,235,788 - PS417_05490 0.53 +0.8 1,235,788 - PS417_05490 0.53 -0.9 1,235,988 + PS417_05490 0.76 -0.8 1,236,266 + +0.6 1,236,269 - -0.4 1,236,653 - PS417_05495 0.52 -2.9 1,236,653 - PS417_05495 0.52 +0.8 1,236,653 - PS417_05495 0.52 -0.4 1,236,653 - PS417_05495 0.52 -0.6 1,236,679 + PS417_05495 0.55 +0.2 1,236,763 + PS417_05495 0.66 +3.0 1,236,764 - PS417_05495 0.66 -0.1 1,236,770 + PS417_05495 0.67 +2.9 1,236,771 - PS417_05495 0.67 +1.7 1,236,773 - PS417_05495 0.67 +0.9 1,236,773 - PS417_05495 0.67 -1.4 1,236,866 - PS417_05495 0.79 +2.6 1,237,047 + +1.3 1,237,047 + -0.8 1,237,047 + -0.6 1,237,111 - -1.4
Or see this region's nucleotide sequence