Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03775 and PS417_03780 overlap by 47 nucleotides PS417_03780 and PS417_03785 are separated by 192 nucleotides PS417_03785 and PS417_03790 overlap by 4 nucleotides
PS417_03775: PS417_03775 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 855,075 to 856,022
_03775
PS417_03780: PS417_03780 - general secretion pathway protein GspH, at 855,976 to 856,557
_03780
PS417_03785: PS417_03785 - pilus assembly protein PilV, at 856,750 to 857,235
_03785
PS417_03790: PS417_03790 - pilus assembly protein PilW, at 857,232 to 857,939
_03790
Position (kb) 855
856
857 Strain fitness (log2 ratio) -1
0
1 at 855.008 kb on + strand at 855.024 kb on + strand at 855.024 kb on + strand at 856.025 kb on - strand at 856.144 kb on + strand, within PS417_03780 at 856.144 kb on + strand, within PS417_03780 at 856.145 kb on - strand, within PS417_03780 at 856.557 kb on + strand at 856.558 kb on - strand at 856.558 kb on - strand at 856.594 kb on + strand at 856.594 kb on + strand at 856.594 kb on + strand at 856.595 kb on - strand at 856.595 kb on - strand at 856.595 kb on - strand at 856.597 kb on - strand at 856.641 kb on + strand at 856.642 kb on - strand at 856.685 kb on + strand at 856.895 kb on - strand, within PS417_03785 at 856.939 kb on + strand, within PS417_03785 at 857.327 kb on + strand, within PS417_03790
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 855,008 + -0.1 855,024 + +1.7 855,024 + -0.4 856,025 - -0.3 856,144 + PS417_03780 0.29 +0.5 856,144 + PS417_03780 0.29 +0.8 856,145 - PS417_03780 0.29 +1.4 856,557 + -1.2 856,558 - +0.7 856,558 - -0.8 856,594 + +0.3 856,594 + -0.5 856,594 + -0.5 856,595 - +0.7 856,595 - +0.9 856,595 - -0.2 856,597 - +0.1 856,641 + -0.1 856,642 - +1.7 856,685 + +0.6 856,895 - PS417_03785 0.30 +0.3 856,939 + PS417_03785 0.39 -0.2 857,327 + PS417_03790 0.13 +0.1
Or see this region's nucleotide sequence