Experiment: JP1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16055 and PS417_16060 are separated by 15 nucleotides PS417_16060 and PS417_16065 are separated by 46 nucleotides PS417_16065 and PS417_16070 are separated by 65 nucleotides
PS417_16055: PS417_16055 - biopolymer transporter TolR, at 3,518,433 to 3,518,879
_16055
PS417_16060: PS417_16060 - protein tolQ, at 3,518,895 to 3,519,587
_16060
PS417_16065: PS417_16065 - 3-phytase, at 3,519,634 to 3,521,499
_16065
PS417_16070: PS417_16070 - TonB-dependent receptor, at 3,521,565 to 3,524,078
_16070
Position (kb)
3519
3520
3521
3522 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3518.975 kb on - strand, within PS417_16060 at 3519.178 kb on + strand, within PS417_16060 at 3519.178 kb on + strand at 3519.179 kb on - strand, within PS417_16060 at 3519.313 kb on + strand, within PS417_16060 at 3519.566 kb on + strand at 3519.566 kb on + strand at 3519.567 kb on - strand at 3519.567 kb on - strand at 3519.636 kb on - strand at 3519.881 kb on + strand, within PS417_16065 at 3519.881 kb on + strand at 3519.915 kb on - strand, within PS417_16065 at 3520.454 kb on + strand, within PS417_16065 at 3521.162 kb on + strand, within PS417_16065 at 3521.163 kb on - strand, within PS417_16065 at 3521.476 kb on + strand at 3521.477 kb on - strand at 3521.477 kb on - strand at 3521.477 kb on - strand at 3521.477 kb on - strand at 3521.597 kb on - strand at 3521.656 kb on + strand at 3521.657 kb on - strand at 3521.812 kb on + strand at 3521.812 kb on + strand at 3521.972 kb on + strand, within PS417_16070 at 3522.076 kb on + strand, within PS417_16070 at 3522.077 kb on - strand, within PS417_16070 at 3522.080 kb on + strand, within PS417_16070 at 3522.081 kb on - strand, within PS417_16070 at 3522.081 kb on - strand, within PS417_16070
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 10 remove 3,518,975 - PS417_16060 0.12 -1.6 3,519,178 + PS417_16060 0.41 -1.6 3,519,178 + -0.0 3,519,179 - PS417_16060 0.41 +0.6 3,519,313 + PS417_16060 0.60 -2.6 3,519,566 + -0.0 3,519,566 + +2.1 3,519,567 - -0.5 3,519,567 - -0.9 3,519,636 - -1.5 3,519,881 + PS417_16065 0.13 +0.2 3,519,881 + -1.0 3,519,915 - PS417_16065 0.15 +1.1 3,520,454 + PS417_16065 0.44 +1.6 3,521,162 + PS417_16065 0.82 +0.5 3,521,163 - PS417_16065 0.82 -0.0 3,521,476 + +3.3 3,521,477 - +1.7 3,521,477 - -1.5 3,521,477 - -0.3 3,521,477 - -0.0 3,521,597 - +0.2 3,521,656 + -0.4 3,521,657 - -1.0 3,521,812 + -0.4 3,521,812 + -0.2 3,521,972 + PS417_16070 0.16 -1.0 3,522,076 + PS417_16070 0.20 -0.4 3,522,077 - PS417_16070 0.20 +1.6 3,522,080 + PS417_16070 0.20 -0.8 3,522,081 - PS417_16070 0.21 +0.1 3,522,081 - PS417_16070 0.21 -0.8
Or see this region's nucleotide sequence