Experiment: P2L5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0745 and Atu0746 are separated by 89 nucleotides Atu0746 and Atu0747 are separated by 260 nucleotides Atu0747 and Atu0748 are separated by 2 nucleotides
Atu0745: Atu0745 - 1-deoxy-D-xylulose-5-phosphate synthase, at 742,737 to 744,656
Atu0745
Atu0746: Atu0746 - hypothetical protein, at 744,746 to 745,669
Atu0746
Atu0747: Atu0747 - exodeoxyribonuclease small subunit, at 745,930 to 746,184
Atu0747
Atu0748: Atu0748 - deacetylase, at 746,187 to 747,122
Atu0748
Position (kb) 744
745
746 Strain fitness (log2 ratio) -2
-1
0
1 at 744.712 kb on - strand at 744.724 kb on - strand at 744.810 kb on + strand at 744.819 kb on + strand at 744.837 kb on - strand at 745.219 kb on - strand, within Atu0746 at 745.257 kb on - strand, within Atu0746 at 745.272 kb on + strand, within Atu0746 at 745.282 kb on + strand, within Atu0746 at 745.290 kb on - strand, within Atu0746 at 745.612 kb on - strand at 745.665 kb on + strand at 745.725 kb on + strand at 745.796 kb on - strand at 745.816 kb on - strand at 745.816 kb on - strand at 745.841 kb on - strand at 746.069 kb on + strand, within Atu0747 at 746.185 kb on + strand at 746.352 kb on - strand, within Atu0748 at 746.503 kb on + strand, within Atu0748 at 746.582 kb on + strand, within Atu0748 at 746.622 kb on - strand, within Atu0748 at 746.654 kb on + strand, within Atu0748 at 746.662 kb on - strand, within Atu0748
Per-strain Table
Position Strand Gene LocusTag Fraction P2L5 remove 744,712 - +0.5 744,724 - +0.8 744,810 + +0.3 744,819 + -0.6 744,837 - -1.1 745,219 - Atu0746 0.51 -1.2 745,257 - Atu0746 0.55 +1.5 745,272 + Atu0746 0.57 +0.5 745,282 + Atu0746 0.58 -0.3 745,290 - Atu0746 0.59 -0.7 745,612 - +0.1 745,665 + -1.1 745,725 + +0.4 745,796 - -0.2 745,816 - -1.5 745,816 - -2.5 745,841 - +0.1 746,069 + Atu0747 0.55 -0.5 746,185 + -2.0 746,352 - Atu0748 0.18 -0.4 746,503 + Atu0748 0.34 -0.3 746,582 + Atu0748 0.42 +0.8 746,622 - Atu0748 0.46 +1.2 746,654 + Atu0748 0.50 +1.5 746,662 - Atu0748 0.51 -0.4
Or see this region's nucleotide sequence