Strain Fitness in Pseudomonas putida KT2440 around PP_0839

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0838 and PP_0839 are separated by 151 nucleotidesPP_0839 and PP_0840 are separated by 3 nucleotidesPP_0840 and PP_0841 are separated by 179 nucleotides PP_0838: PP_0838 - inositol monophosphatase / glycerol-2-phosphatase, at 975,320 to 976,138 _0838 PP_0839: PP_0839 - tRNA Cm32/Um32 methyltransferase, at 976,290 to 977,045 _0839 PP_0840: PP_0840 - serine acetyltransferase, at 977,049 to 977,834 _0840 PP_0841: PP_0841 - DNA-binding transcriptional dual regulator 2Fe-2S cluster IscR, at 978,014 to 978,505 _0841 Position (kb) 976 977 978Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 976.230 kb on + strandat 976.248 kb on - strandat 976.302 kb on - strandat 976.322 kb on + strandat 976.508 kb on + strand, within PP_0839at 976.854 kb on + strand, within PP_0839at 976.941 kb on + strand, within PP_0839at 976.942 kb on - strand, within PP_0839at 976.942 kb on - strand, within PP_0839at 977.072 kb on + strandat 977.073 kb on - strandat 977.083 kb on + strandat 977.084 kb on - strandat 977.298 kb on - strand, within PP_0840at 977.522 kb on + strand, within PP_0840at 977.588 kb on + strand, within PP_0840at 977.622 kb on + strand, within PP_0840at 977.714 kb on + strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.748 kb on + strand, within PP_0840at 977.748 kb on + strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 978.009 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
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976,230 + -0.4
976,248 - -0.2
976,302 - +0.4
976,322 + -0.5
976,508 + PP_0839 0.29 +0.8
976,854 + PP_0839 0.75 +1.8
976,941 + PP_0839 0.86 +0.5
976,942 - PP_0839 0.86 -1.0
976,942 - PP_0839 0.86 +0.3
977,072 + +1.2
977,073 - -0.9
977,083 + +0.1
977,084 - +0.8
977,298 - PP_0840 0.32 +1.0
977,522 + PP_0840 0.60 -0.1
977,588 + PP_0840 0.69 +0.5
977,622 + PP_0840 0.73 +0.1
977,714 + PP_0840 0.85 -1.5
977,715 - PP_0840 0.85 -2.3
977,715 - PP_0840 0.85 -0.4
977,715 - PP_0840 0.85 -0.3
977,715 - PP_0840 0.85 +0.2
977,748 + PP_0840 0.89 +0.2
977,748 + PP_0840 0.89 -0.0
977,749 - PP_0840 0.89 +1.4
977,749 - PP_0840 0.89 -0.5
977,749 - PP_0840 0.89 -2.8
977,749 - PP_0840 0.89 +0.4
978,009 + +0.3

Or see this region's nucleotide sequence