Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0006 and PP_0007 are separated by 2 nucleotides PP_0007 and PP_0008 overlap by 8 nucleotides PP_0008 and PP_0009 are separated by 13 nucleotides
PP_0006: PP_0006 - probable membrane protein insertase, at 6,471 to 8,153
_0006
PP_0007: PP_0007 - conserved hypothetical protein, at 8,156 to 8,401
_0007
PP_0008: PP_0008 - ribonuclease P protein component, at 8,394 to 8,798
_0008
PP_0009: PP_0009 - 50S ribosomal protein L34, at 8,812 to 8,946
_0009
Position (kb)
8
9 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 8.209 kb on - strand, within PP_0007 at 8.209 kb on - strand, within PP_0007 at 8.299 kb on - strand at 8.299 kb on - strand, within PP_0007 at 8.354 kb on - strand, within PP_0007 at 8.396 kb on - strand at 9.056 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.152 kb on + strand at 9.154 kb on + strand at 9.154 kb on + strand at 9.155 kb on - strand at 9.177 kb on - strand at 9.186 kb on - strand at 9.238 kb on + strand at 9.238 kb on + strand at 9.249 kb on - strand at 9.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs remove 8,209 - PP_0007 0.22 -1.6 8,209 - PP_0007 0.22 -1.5 8,299 - -0.0 8,299 - PP_0007 0.58 +0.4 8,354 - PP_0007 0.80 -3.8 8,396 - -3.4 9,056 - -0.7 9,058 - +0.6 9,058 - +0.2 9,058 - +1.1 9,058 - -2.3 9,152 + -0.8 9,154 + -0.0 9,154 + +0.4 9,155 - +0.0 9,177 - +0.1 9,186 - -0.5 9,238 + -0.8 9,238 + +0.4 9,249 - -2.2 9,395 - -0.0
Or see this region's nucleotide sequence