Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0620 and PP_0621 are separated by 266 nucleotides PP_0621 and PP_0622 are separated by 13 nucleotides PP_0622 and PP_0623 are separated by 147 nucleotides
PP_0620: PP_0620 - GntR family transcriptional regulator, at 725,989 to 726,753
_0620
PP_0621: PP_0621 - conserved protein of unknown function, at 727,020 to 727,331
_0621
PP_0622: PP_0622 - Outer membrane protein assembly factor BamD, at 727,345 to 728,364
_0622
PP_0623: PP_0623 - 23S rRNA pseudouridine(1911,1915,1917) synthase, at 728,512 to 729,474
_0623
Position (kb) 727
728
729 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 726.377 kb on + strand, within PP_0620 at 726.446 kb on + strand, within PP_0620 at 726.446 kb on + strand, within PP_0620 at 726.447 kb on - strand, within PP_0620 at 726.447 kb on - strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.483 kb on - strand, within PP_0620 at 726.839 kb on + strand at 726.840 kb on - strand at 726.840 kb on - strand at 726.840 kb on - strand at 726.867 kb on + strand at 727.066 kb on + strand, within PP_0621 at 727.066 kb on + strand, within PP_0621 at 727.066 kb on + strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.189 kb on + strand, within PP_0621 at 727.189 kb on + strand, within PP_0621 at 727.190 kb on - strand, within PP_0621 at 727.370 kb on + strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.444 kb on - strand at 727.643 kb on + strand, within PP_0622 at 728.416 kb on - strand at 728.416 kb on - strand at 728.416 kb on - strand at 728.771 kb on - strand, within PP_0623 at 729.019 kb on + strand, within PP_0623 at 729.019 kb on + strand, within PP_0623 at 729.266 kb on - strand, within PP_0623 at 729.283 kb on - strand, within PP_0623
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs remove 726,377 + PP_0620 0.51 -2.9 726,446 + PP_0620 0.60 +2.1 726,446 + PP_0620 0.60 +1.2 726,447 - PP_0620 0.60 -4.1 726,447 - PP_0620 0.60 -0.5 726,482 + PP_0620 0.64 -0.0 726,482 + PP_0620 0.64 +3.8 726,482 + PP_0620 0.64 -3.8 726,482 + PP_0620 0.64 +0.7 726,483 - PP_0620 0.65 -3.2 726,839 + +0.3 726,840 - -1.1 726,840 - +1.5 726,840 - -1.7 726,867 + -3.3 727,066 + PP_0621 0.15 -0.3 727,066 + PP_0621 0.15 -2.8 727,066 + PP_0621 0.15 -0.5 727,067 - PP_0621 0.15 -2.7 727,067 - PP_0621 0.15 +1.1 727,067 - PP_0621 0.15 -3.2 727,189 + PP_0621 0.54 -0.5 727,189 + PP_0621 0.54 -4.9 727,190 - PP_0621 0.54 -0.6 727,370 + -0.4 727,371 - -3.5 727,371 - -0.8 727,371 - -0.2 727,371 - -0.5 727,444 - -0.8 727,643 + PP_0622 0.29 -0.6 728,416 - +2.0 728,416 - -0.6 728,416 - +0.4 728,771 - PP_0623 0.27 +1.0 729,019 + PP_0623 0.53 -1.2 729,019 + PP_0623 0.53 +1.0 729,266 - PP_0623 0.78 -0.0 729,283 - PP_0623 0.80 -0.0
Or see this region's nucleotide sequence