Strain Fitness in Pseudomonas putida KT2440 around PP_4916

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4915 and PP_4916 are separated by 35 nucleotidesPP_4916 and PP_4917 are separated by 131 nucleotides PP_4915: PP_4915 - DNA topoisomerase IV subunit B, at 5,587,243 to 5,589,147 _4915 PP_4916: PP_4916 - putative esterase, at 5,589,183 to 5,589,791 _4916 PP_4917: PP_4917 - 3',5'-cyclic-nucleotide phosphodiesterase, at 5,589,923 to 5,590,723 _4917 Position (kb) 5589 5590Strain fitness (log2 ratio) -2 -1 0 1 2at 5589.212 kb on - strandat 5589.322 kb on + strand, within PP_4916at 5589.323 kb on - strand, within PP_4916at 5589.466 kb on + strand, within PP_4916at 5589.466 kb on + strand, within PP_4916at 5589.574 kb on + strand, within PP_4916at 5589.574 kb on + strand, within PP_4916at 5589.574 kb on + strand, within PP_4916at 5589.574 kb on + strand, within PP_4916at 5589.574 kb on + strand, within PP_4916at 5589.574 kb on + strand, within PP_4916at 5589.575 kb on - strand, within PP_4916at 5589.575 kb on - strand, within PP_4916at 5589.770 kb on + strandat 5589.771 kb on - strandat 5589.771 kb on - strandat 5589.772 kb on + strandat 5589.772 kb on + strandat 5589.772 kb on + strandat 5589.772 kb on + strandat 5589.772 kb on + strandat 5589.773 kb on - strandat 5589.825 kb on - strandat 5589.927 kb on + strandat 5590.151 kb on + strand, within PP_4917at 5590.152 kb on - strand, within PP_4917at 5590.152 kb on - strand, within PP_4917at 5590.163 kb on + strand, within PP_4917at 5590.164 kb on - strand, within PP_4917at 5590.164 kb on - strand, within PP_4917at 5590.186 kb on + strand, within PP_4917at 5590.518 kb on + strand, within PP_4917at 5590.518 kb on + strand, within PP_4917at 5590.518 kb on + strand, within PP_4917at 5590.519 kb on - strand, within PP_4917at 5590.519 kb on - strand, within PP_4917at 5590.519 kb on - strand, within PP_4917at 5590.519 kb on - strand, within PP_4917at 5590.519 kb on - strand, within PP_4917at 5590.519 kb on - strand, within PP_4917at 5590.756 kb on + strandat 5590.756 kb on + strandat 5590.757 kb on - strandat 5590.757 kb on - strandat 5590.757 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
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5,589,212 - +0.0
5,589,322 + PP_4916 0.23 -0.5
5,589,323 - PP_4916 0.23 -0.4
5,589,466 + PP_4916 0.46 +1.5
5,589,466 + PP_4916 0.46 +0.1
5,589,574 + PP_4916 0.64 -0.7
5,589,574 + PP_4916 0.64 +0.8
5,589,574 + PP_4916 0.64 +0.2
5,589,574 + PP_4916 0.64 +0.1
5,589,574 + PP_4916 0.64 +0.7
5,589,574 + PP_4916 0.64 +0.7
5,589,575 - PP_4916 0.64 +1.0
5,589,575 - PP_4916 0.64 +0.8
5,589,770 + -0.3
5,589,771 - -0.2
5,589,771 - +1.0
5,589,772 + -1.5
5,589,772 + -0.2
5,589,772 + -0.4
5,589,772 + +0.4
5,589,772 + -0.8
5,589,773 - -2.3
5,589,825 - +1.5
5,589,927 + +0.3
5,590,151 + PP_4917 0.28 +0.5
5,590,152 - PP_4917 0.29 +0.3
5,590,152 - PP_4917 0.29 -0.2
5,590,163 + PP_4917 0.30 +0.2
5,590,164 - PP_4917 0.30 -1.2
5,590,164 - PP_4917 0.30 -0.2
5,590,186 + PP_4917 0.33 +1.8
5,590,518 + PP_4917 0.74 -1.0
5,590,518 + PP_4917 0.74 -0.6
5,590,518 + PP_4917 0.74 -0.5
5,590,519 - PP_4917 0.74 +0.6
5,590,519 - PP_4917 0.74 -0.6
5,590,519 - PP_4917 0.74 -0.4
5,590,519 - PP_4917 0.74 -0.6
5,590,519 - PP_4917 0.74 -1.1
5,590,519 - PP_4917 0.74 +1.3
5,590,756 + -0.8
5,590,756 + -1.9
5,590,757 - +0.3
5,590,757 - +0.1
5,590,757 - +0.5

Or see this region's nucleotide sequence