Strain Fitness in Pseudomonas putida KT2440 around PP_0122

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0121 and PP_0122 are separated by 69 nucleotidesPP_0122 and PP_0123 are separated by 75 nucleotides PP_0121: PP_0121 - homoserine kinase, at 126,140 to 127,090 _0121 PP_0122: PP_0122 - conserved exported protein of unknown function, at 127,160 to 127,447 _0122 PP_0123: PP_0123 - DNA polymerase I, 5, at 127,523 to 130,270 _0123 Position (kb) 127 128Strain fitness (log2 ratio) -1 0 1at 126.345 kb on + strandat 126.629 kb on + strandat 127.162 kb on - strandat 127.291 kb on - strand, within PP_0122at 127.291 kb on - strand, within PP_0122at 127.312 kb on - strand, within PP_0122at 127.327 kb on + strand, within PP_0122at 127.328 kb on - strand, within PP_0122at 127.424 kb on + strandat 127.425 kb on - strandat 127.450 kb on + strandat 127.455 kb on + strandat 127.455 kb on + strandat 127.455 kb on + strandat 127.456 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
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126,345 + -0.7
126,629 + +0.7
127,162 - +1.6
127,291 - PP_0122 0.45 +0.5
127,291 - PP_0122 0.45 +0.2
127,312 - PP_0122 0.53 +0.1
127,327 + PP_0122 0.58 -1.1
127,328 - PP_0122 0.58 +1.6
127,424 + -0.1
127,425 - +0.2
127,450 + +0.9
127,455 + +0.7
127,455 + -0.4
127,455 + -1.6
127,456 - +1.0

Or see this region's nucleotide sequence