Strain Fitness in Pseudomonas putida KT2440 around PP_5541

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2939 and PP_2940 are separated by 19 nucleotidesPP_2940 and PP_5541 are separated by 591 nucleotidesPP_5541 and PP_5542 are separated by 74 nucleotidesPP_5542 and PP_2941 are separated by 71 nucleotides PP_2939: PP_2939 - toxin of the YefM-YoeB antitoxin/toxin complex and DNA-binding transcriptional repressor, at 3,342,448 to 3,342,702 _2939 PP_2940: PP_2940 - antitoxin of the YoeB-YefM toxin-antitoxin pair and DNA binding transcriptional repressor, at 3,342,722 to 3,343,006 _2940 PP_5541: PP_5541 - conserved protein of unknown function, at 3,343,598 to 3,343,927 _5541 PP_5542: PP_5542 - protein of unknown function, at 3,344,002 to 3,344,271 _5542 PP_2941: PP_2941 - conserved protein of unknown function, at 3,344,343 to 3,345,128 _2941 Position (kb) 3343 3344Strain fitness (log2 ratio) -2 -1 0 1at 3342.605 kb on - strand, within PP_2939at 3342.619 kb on + strand, within PP_2939at 3342.671 kb on + strand, within PP_2939at 3342.671 kb on + strand, within PP_2939at 3342.671 kb on + strandat 3342.672 kb on - strand, within PP_2939at 3342.706 kb on + strandat 3342.706 kb on + strandat 3342.706 kb on + strandat 3342.706 kb on + strandat 3342.707 kb on - strandat 3342.707 kb on - strandat 3342.707 kb on - strandat 3343.226 kb on - strandat 3343.269 kb on + strandat 3343.269 kb on + strandat 3343.270 kb on - strandat 3343.302 kb on + strandat 3343.303 kb on - strandat 3343.338 kb on - strandat 3343.339 kb on + strandat 3343.340 kb on - strandat 3343.486 kb on + strandat 3343.486 kb on + strandat 3343.504 kb on - strandat 3343.525 kb on + strandat 3343.525 kb on + strandat 3343.555 kb on - strandat 3343.586 kb on + strandat 3343.659 kb on - strand, within PP_5541at 3343.741 kb on + strand, within PP_5541at 3343.741 kb on + strand, within PP_5541at 3343.746 kb on - strand, within PP_5541at 3343.779 kb on + strand, within PP_5541at 3343.780 kb on - strand, within PP_5541at 3343.780 kb on - strand, within PP_5541at 3343.780 kb on - strand, within PP_5541at 3343.970 kb on + strandat 3343.970 kb on + strandat 3343.971 kb on - strandat 3344.045 kb on - strand, within PP_5542at 3344.045 kb on - strand, within PP_5542at 3344.056 kb on + strand, within PP_5542at 3344.057 kb on - strand, within PP_5542at 3344.066 kb on - strand, within PP_5542at 3344.133 kb on - strand, within PP_5542at 3344.231 kb on - strand, within PP_5542at 3344.231 kb on - strand, within PP_5542at 3344.410 kb on + strandat 3344.411 kb on - strandat 3344.545 kb on - strand, within PP_2941at 3344.545 kb on - strand, within PP_2941at 3344.656 kb on + strand, within PP_2941at 3344.656 kb on + strand, within PP_2941at 3344.657 kb on - strand, within PP_2941at 3344.657 kb on - strand, within PP_2941at 3344.660 kb on - strand, within PP_2941at 3344.660 kb on - strand, within PP_2941at 3344.698 kb on + strand, within PP_2941at 3344.699 kb on - strand, within PP_2941at 3344.903 kb on - strand, within PP_2941

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
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3,342,605 - PP_2939 0.62 +0.3
3,342,619 + PP_2939 0.67 +0.4
3,342,671 + PP_2939 0.87 -0.1
3,342,671 + PP_2939 0.87 -0.0
3,342,671 + +0.1
3,342,672 - PP_2939 0.88 -0.4
3,342,706 + +0.3
3,342,706 + +1.0
3,342,706 + -0.6
3,342,706 + -0.2
3,342,707 - -0.6
3,342,707 - -0.0
3,342,707 - -0.7
3,343,226 - -0.7
3,343,269 + +0.3
3,343,269 + +0.0
3,343,270 - -0.6
3,343,302 + +1.3
3,343,303 - -0.8
3,343,338 - -0.0
3,343,339 + +0.5
3,343,340 - +0.0
3,343,486 + -0.6
3,343,486 + -0.0
3,343,504 - +0.3
3,343,525 + -0.1
3,343,525 + -0.2
3,343,555 - -0.6
3,343,586 + +0.6
3,343,659 - PP_5541 0.18 +0.2
3,343,741 + PP_5541 0.43 +1.1
3,343,741 + PP_5541 0.43 +0.8
3,343,746 - PP_5541 0.45 -0.1
3,343,779 + PP_5541 0.55 +1.2
3,343,780 - PP_5541 0.55 -0.3
3,343,780 - PP_5541 0.55 +0.7
3,343,780 - PP_5541 0.55 -0.8
3,343,970 + -1.0
3,343,970 + +0.0
3,343,971 - -0.4
3,344,045 - PP_5542 0.16 +0.3
3,344,045 - PP_5542 0.16 +0.9
3,344,056 + PP_5542 0.20 +0.1
3,344,057 - PP_5542 0.20 +0.5
3,344,066 - PP_5542 0.24 +0.8
3,344,133 - PP_5542 0.49 +0.0
3,344,231 - PP_5542 0.85 -1.0
3,344,231 - PP_5542 0.85 +0.1
3,344,410 + +0.3
3,344,411 - -1.0
3,344,545 - PP_2941 0.26 +0.9
3,344,545 - PP_2941 0.26 +0.1
3,344,656 + PP_2941 0.40 +0.6
3,344,656 + PP_2941 0.40 +0.5
3,344,657 - PP_2941 0.40 +0.0
3,344,657 - PP_2941 0.40 -0.1
3,344,660 - PP_2941 0.40 -0.2
3,344,660 - PP_2941 0.40 -0.4
3,344,698 + PP_2941 0.45 -0.2
3,344,699 - PP_2941 0.45 +0.1
3,344,903 - PP_2941 0.71 -1.9

Or see this region's nucleotide sequence