Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_t73 and PP_0277 are separated by 98 nucleotides PP_0277 and PP_5437 are separated by 203 nucleotides PP_5437 and PP_0278 are separated by 134 nucleotides PP_0278 and PP_0279 are separated by 392 nucleotides
PP_t73: PP_t73 - tRNA-Thr, at 334,973 to 335,048
_t73
PP_0277: PP_0277 - conserved exported protein of unknown function, at 335,147 to 335,395
_0277
PP_5437: PP_5437 - protein of unknown function, at 335,599 to 335,781
_5437
PP_0278: PP_0278 - conserved protein of unknown function, at 335,916 to 336,152
_0278
PP_0279: PP_0279 - conserved protein of unknown function, at 336,545 to 337,750
_0279
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1 at 334.965 kb on - strand at 334.971 kb on + strand at 334.972 kb on - strand at 334.972 kb on - strand at 335.065 kb on - strand at 335.119 kb on - strand at 335.505 kb on + strand at 335.505 kb on + strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.548 kb on - strand at 335.548 kb on - strand at 335.548 kb on - strand at 335.577 kb on - strand at 335.600 kb on + strand at 335.601 kb on - strand at 335.608 kb on + strand at 335.663 kb on + strand, within PP_5437 at 335.664 kb on - strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.669 kb on - strand, within PP_5437 at 335.669 kb on - strand, within PP_5437 at 335.686 kb on - strand, within PP_5437 at 335.686 kb on - strand, within PP_5437 at 335.765 kb on + strand at 335.765 kb on + strand at 335.765 kb on + strand at 335.765 kb on + strand at 335.765 kb on + strand at 335.766 kb on - strand at 335.766 kb on - strand at 335.790 kb on + strand at 335.790 kb on + strand at 335.790 kb on + strand at 335.791 kb on - strand at 335.825 kb on - strand at 335.850 kb on - strand at 335.998 kb on + strand, within PP_0278 at 336.004 kb on + strand, within PP_0278 at 336.005 kb on - strand, within PP_0278 at 336.005 kb on - strand, within PP_0278 at 336.033 kb on + strand, within PP_0278 at 336.105 kb on - strand, within PP_0278 at 336.125 kb on + strand, within PP_0278 at 336.178 kb on + strand at 336.188 kb on + strand at 336.188 kb on + strand at 336.188 kb on + strand at 336.403 kb on + strand at 336.500 kb on + strand at 336.501 kb on - strand at 336.524 kb on + strand at 336.624 kb on + strand at 336.624 kb on + strand at 336.625 kb on - strand at 336.813 kb on + strand, within PP_0279 at 336.813 kb on + strand, within PP_0279 at 336.813 kb on + strand, within PP_0279 at 336.813 kb on + strand, within PP_0279 at 336.814 kb on - strand, within PP_0279 at 336.814 kb on - strand, within PP_0279 at 336.867 kb on + strand, within PP_0279 at 336.868 kb on - strand, within PP_0279 at 337.047 kb on + strand, within PP_0279 at 337.071 kb on + strand, within PP_0279 at 337.072 kb on - strand, within PP_0279 at 337.072 kb on - strand, within PP_0279 at 337.133 kb on + strand, within PP_0279 at 337.133 kb on + strand, within PP_0279 at 337.134 kb on - strand, within PP_0279 at 337.134 kb on - strand, within PP_0279
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs remove 334,965 - -0.6 334,971 + -0.8 334,972 - -0.7 334,972 - +0.3 335,065 - -1.0 335,119 - +0.4 335,505 + -0.0 335,505 + -0.2 335,506 - -0.2 335,506 - +0.5 335,506 - -0.7 335,506 - -0.9 335,506 - +0.9 335,548 - -0.3 335,548 - +0.8 335,548 - +0.1 335,577 - -1.0 335,600 + -2.3 335,601 - -0.1 335,608 + -0.9 335,663 + PP_5437 0.35 +0.5 335,664 - PP_5437 0.36 +0.3 335,668 + PP_5437 0.38 +0.5 335,668 + PP_5437 0.38 +0.3 335,668 + PP_5437 0.38 +0.5 335,669 - PP_5437 0.38 -0.3 335,669 - PP_5437 0.38 +0.0 335,686 - PP_5437 0.48 -0.6 335,686 - PP_5437 0.48 -0.7 335,765 + -1.0 335,765 + +0.5 335,765 + +0.2 335,765 + +0.7 335,765 + -0.1 335,766 - +0.0 335,766 - +0.5 335,790 + +0.4 335,790 + -0.8 335,790 + -0.4 335,791 - +0.1 335,825 - -0.2 335,850 - -0.5 335,998 + PP_0278 0.35 +0.3 336,004 + PP_0278 0.37 +0.8 336,005 - PP_0278 0.38 +0.0 336,005 - PP_0278 0.38 +1.0 336,033 + PP_0278 0.49 -0.3 336,105 - PP_0278 0.80 +0.0 336,125 + PP_0278 0.88 +0.5 336,178 + +0.2 336,188 + -0.7 336,188 + +0.1 336,188 + +0.4 336,403 + +0.6 336,500 + -1.3 336,501 - -1.2 336,524 + -0.1 336,624 + -0.3 336,624 + -0.1 336,625 - +1.1 336,813 + PP_0279 0.22 -1.7 336,813 + PP_0279 0.22 -0.6 336,813 + PP_0279 0.22 -0.1 336,813 + PP_0279 0.22 +0.7 336,814 - PP_0279 0.22 +0.1 336,814 - PP_0279 0.22 +0.8 336,867 + PP_0279 0.27 +0.4 336,868 - PP_0279 0.27 -0.9 337,047 + PP_0279 0.42 +0.6 337,071 + PP_0279 0.44 +0.0 337,072 - PP_0279 0.44 +0.6 337,072 - PP_0279 0.44 +0.7 337,133 + PP_0279 0.49 +1.0 337,133 + PP_0279 0.49 -0.5 337,134 - PP_0279 0.49 -1.4 337,134 - PP_0279 0.49 +0.5
Or see this region's nucleotide sequence