Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2299 and PP_2300 are separated by 91 nucleotides PP_2300 and PP_2301 are separated by 63 nucleotides PP_2301 and PP_2302 are separated by 163 nucleotides
PP_2299: PP_2299 - Trigger factor, at 2,626,916 to 2,628,247
_2299
PP_2300: PP_2300 - ATP-dependent Clp protease proteolytic subunit, at 2,628,339 to 2,628,980
_2300
PP_2301: PP_2301 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,629,044 to 2,630,372
_2301
PP_2302: PP_2302 - DNA-binding, ATP-dependent protease, at 2,630,536 to 2,632,932
_2302
Position (kb)
2629
2630
2631 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2628.214 kb on + strand at 2628.302 kb on + strand at 2628.302 kb on + strand at 2628.317 kb on + strand at 2629.368 kb on + strand at 2629.369 kb on - strand, within PP_2301 at 2629.605 kb on + strand, within PP_2301 at 2630.244 kb on + strand at 2630.244 kb on + strand at 2630.244 kb on + strand at 2630.244 kb on + strand at 2630.453 kb on - strand at 2630.477 kb on + strand at 2630.477 kb on + strand at 2630.477 kb on + strand at 2630.477 kb on + strand at 2630.478 kb on - strand at 2630.675 kb on + strand at 2630.675 kb on + strand at 2630.676 kb on - strand at 2630.676 kb on - strand at 2630.676 kb on - strand at 2630.945 kb on - strand, within PP_2302 at 2630.945 kb on - strand, within PP_2302
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs remove 2,628,214 + -1.6 2,628,302 + +0.4 2,628,302 + -2.7 2,628,317 + -0.3 2,629,368 + +2.6 2,629,369 - PP_2301 0.24 +2.6 2,629,605 + PP_2301 0.42 +1.6 2,630,244 + +1.0 2,630,244 + +1.0 2,630,244 + +1.0 2,630,244 + +1.6 2,630,453 - -0.9 2,630,477 + +0.2 2,630,477 + -2.0 2,630,477 + -0.0 2,630,477 + -0.8 2,630,478 - +0.3 2,630,675 + -1.1 2,630,675 + -1.0 2,630,676 - +0.6 2,630,676 - +1.6 2,630,676 - +0.3 2,630,945 - PP_2302 0.17 +0.3 2,630,945 - PP_2302 0.17 -1.3
Or see this region's nucleotide sequence