Strain Fitness in Pseudomonas putida KT2440 around PP_1272

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1271 and PP_1272 overlap by 14 nucleotidesPP_1272 and PP_1273 overlap by 4 nucleotides PP_1271: PP_1271 - putative Multidrug efflux MFS transporter, at 1,451,839 to 1,453,380 _1271 PP_1272: PP_1272 - putative Multidrug efflux MFS membrane fusion protein, at 1,453,367 to 1,454,470 _1272 PP_1273: PP_1273 - putative Multidrug efflux MFS outer membrane protein, at 1,454,467 to 1,455,921 _1273 Position (kb) 1453 1454 1455Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1452.379 kb on + strand, within PP_1271at 1452.640 kb on + strand, within PP_1271at 1452.988 kb on + strand, within PP_1271at 1452.997 kb on + strand, within PP_1271at 1452.997 kb on + strand, within PP_1271at 1453.271 kb on + strandat 1453.272 kb on - strandat 1453.292 kb on - strandat 1453.292 kb on - strandat 1453.526 kb on + strand, within PP_1272at 1453.527 kb on - strand, within PP_1272at 1453.527 kb on - strand, within PP_1272at 1453.527 kb on - strand, within PP_1272at 1453.527 kb on - strand, within PP_1272at 1453.541 kb on + strand, within PP_1272at 1453.542 kb on - strand, within PP_1272at 1453.542 kb on - strand, within PP_1272at 1453.650 kb on - strand, within PP_1272at 1453.689 kb on + strand, within PP_1272at 1453.690 kb on - strand, within PP_1272at 1454.033 kb on + strand, within PP_1272at 1454.033 kb on + strand, within PP_1272at 1454.062 kb on + strand, within PP_1272at 1454.063 kb on - strand, within PP_1272at 1454.097 kb on - strand, within PP_1272at 1454.097 kb on - strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.147 kb on + strand, within PP_1272at 1454.148 kb on - strand, within PP_1272at 1454.148 kb on - strand, within PP_1272at 1454.148 kb on - strand, within PP_1272at 1454.148 kb on - strand, within PP_1272at 1454.148 kb on - strand, within PP_1272at 1454.187 kb on + strand, within PP_1272at 1454.187 kb on + strand, within PP_1272at 1454.187 kb on + strand, within PP_1272at 1454.188 kb on - strand, within PP_1272at 1454.480 kb on + strandat 1454.481 kb on - strandat 1454.752 kb on - strand, within PP_1273at 1454.752 kb on - strand, within PP_1273at 1454.752 kb on - strand, within PP_1273at 1454.811 kb on + strand, within PP_1273at 1455.020 kb on - strand, within PP_1273

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
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1,452,379 + PP_1271 0.35 +0.8
1,452,640 + PP_1271 0.52 -0.6
1,452,988 + PP_1271 0.75 -2.1
1,452,997 + PP_1271 0.75 +0.0
1,452,997 + PP_1271 0.75 +1.6
1,453,271 + -2.6
1,453,272 - -0.8
1,453,292 - -2.5
1,453,292 - +0.5
1,453,526 + PP_1272 0.14 +0.4
1,453,527 - PP_1272 0.14 -3.4
1,453,527 - PP_1272 0.14 +0.9
1,453,527 - PP_1272 0.14 +0.5
1,453,527 - PP_1272 0.14 +1.3
1,453,541 + PP_1272 0.16 -1.9
1,453,542 - PP_1272 0.16 +0.3
1,453,542 - PP_1272 0.16 -0.3
1,453,650 - PP_1272 0.26 +0.4
1,453,689 + PP_1272 0.29 +0.6
1,453,690 - PP_1272 0.29 -1.8
1,454,033 + PP_1272 0.60 -0.4
1,454,033 + PP_1272 0.60 -2.1
1,454,062 + PP_1272 0.63 -1.2
1,454,063 - PP_1272 0.63 -0.2
1,454,097 - PP_1272 0.66 +0.8
1,454,097 - PP_1272 0.66 -0.3
1,454,147 + PP_1272 0.71 +0.4
1,454,147 + PP_1272 0.71 +0.9
1,454,147 + PP_1272 0.71 +0.2
1,454,147 + PP_1272 0.71 -2.0
1,454,147 + PP_1272 0.71 -0.7
1,454,147 + PP_1272 0.71 -0.2
1,454,147 + PP_1272 0.71 +0.4
1,454,148 - PP_1272 0.71 +0.1
1,454,148 - PP_1272 0.71 -2.5
1,454,148 - PP_1272 0.71 -1.6
1,454,148 - PP_1272 0.71 -3.4
1,454,148 - PP_1272 0.71 -3.5
1,454,187 + PP_1272 0.74 -4.4
1,454,187 + PP_1272 0.74 -0.5
1,454,187 + PP_1272 0.74 -3.0
1,454,188 - PP_1272 0.74 -1.5
1,454,480 + +2.2
1,454,481 - +0.3
1,454,752 - PP_1273 0.20 +0.3
1,454,752 - PP_1273 0.20 -4.3
1,454,752 - PP_1273 0.20 -2.5
1,454,811 + PP_1273 0.24 +2.3
1,455,020 - PP_1273 0.38 -0.1

Or see this region's nucleotide sequence