Strain Fitness in Pseudomonas putida KT2440 around PP_5001

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4999 and PP_5000 are separated by 211 nucleotidesPP_5000 and PP_5001 are separated by 76 nucleotidesPP_5001 and PP_5002 are separated by 166 nucleotidesPP_5002 and PP_5003 are separated by 268 nucleotides PP_4999: PP_4999 - dihydroorotase-like protein, at 5,695,277 to 5,696,548 _4999 PP_5000: PP_5000 - peptidase component of the ATP-dependent HslVU protease, at 5,696,760 to 5,697,290 _5000 PP_5001: PP_5001 - protease HslVU, ATPase component, at 5,697,367 to 5,698,710 _5001 PP_5002: PP_5002 - conserved protein of unknown function, at 5,698,877 to 5,699,254 _5002 PP_5003: PP_5003 - poly(3-hydroxyalkanoate) polymerase 1, at 5,699,523 to 5,701,202 _5003 Position (kb) 5697 5698 5699Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5696.511 kb on - strandat 5696.511 kb on - strandat 5696.513 kb on - strandat 5696.550 kb on + strandat 5696.550 kb on + strandat 5696.550 kb on + strandat 5696.551 kb on - strandat 5696.559 kb on + strandat 5696.609 kb on + strandat 5696.609 kb on + strandat 5696.649 kb on + strandat 5696.649 kb on + strandat 5696.650 kb on - strandat 5696.650 kb on - strandat 5696.650 kb on - strandat 5696.650 kb on - strandat 5696.711 kb on + strandat 5696.711 kb on + strandat 5696.712 kb on - strandat 5696.712 kb on - strandat 5697.012 kb on - strand, within PP_5000at 5697.145 kb on - strand, within PP_5000at 5697.360 kb on - strandat 5697.360 kb on - strandat 5697.634 kb on + strand, within PP_5001at 5697.634 kb on + strandat 5697.635 kb on - strand, within PP_5001at 5697.843 kb on - strand, within PP_5001at 5697.843 kb on - strand, within PP_5001at 5698.429 kb on + strand, within PP_5001at 5698.648 kb on + strandat 5698.649 kb on - strandat 5698.700 kb on - strandat 5698.834 kb on - strandat 5698.834 kb on - strandat 5698.834 kb on - strandat 5698.834 kb on - strandat 5698.937 kb on + strand, within PP_5002at 5699.138 kb on + strand, within PP_5002at 5699.139 kb on - strand, within PP_5002at 5699.344 kb on + strandat 5699.344 kb on + strandat 5699.345 kb on - strandat 5699.431 kb on + strandat 5699.449 kb on + strandat 5699.449 kb on + strandat 5699.449 kb on + strandat 5699.493 kb on + strandat 5699.521 kb on - strandat 5699.521 kb on - strandat 5699.528 kb on + strandat 5699.528 kb on + strandat 5699.528 kb on + strandat 5699.529 kb on - strandat 5699.529 kb on - strandat 5699.548 kb on + strandat 5699.548 kb on + strandat 5699.548 kb on + strandat 5699.549 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
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5,696,511 - +2.2
5,696,511 - +2.2
5,696,513 - +2.2
5,696,550 + -0.8
5,696,550 + +0.6
5,696,550 + -1.0
5,696,551 - -0.1
5,696,559 + -0.6
5,696,609 + +1.5
5,696,609 + -2.8
5,696,649 + +0.8
5,696,649 + -0.7
5,696,650 - -0.4
5,696,650 - +0.7
5,696,650 - -1.6
5,696,650 - +0.5
5,696,711 + +0.4
5,696,711 + +0.2
5,696,712 - +1.3
5,696,712 - -3.5
5,697,012 - PP_5000 0.47 +1.1
5,697,145 - PP_5000 0.73 -1.3
5,697,360 - +1.9
5,697,360 - +0.3
5,697,634 + PP_5001 0.20 -0.7
5,697,634 + +0.3
5,697,635 - PP_5001 0.20 -3.0
5,697,843 - PP_5001 0.35 +0.4
5,697,843 - PP_5001 0.35 -2.9
5,698,429 + PP_5001 0.79 -3.5
5,698,648 + -2.7
5,698,649 - +1.1
5,698,700 - -0.0
5,698,834 - +0.9
5,698,834 - +0.5
5,698,834 - +1.4
5,698,834 - +0.4
5,698,937 + PP_5002 0.16 +1.3
5,699,138 + PP_5002 0.69 -0.3
5,699,139 - PP_5002 0.69 -1.7
5,699,344 + -1.0
5,699,344 + +0.3
5,699,345 - +0.1
5,699,431 + -0.1
5,699,449 + -3.2
5,699,449 + +0.2
5,699,449 + -3.4
5,699,493 + +1.4
5,699,521 - -1.3
5,699,521 - -1.4
5,699,528 + +0.3
5,699,528 + -1.1
5,699,528 + +0.5
5,699,529 - +0.1
5,699,529 - +0.2
5,699,548 + +0.5
5,699,548 + -0.1
5,699,548 + -2.3
5,699,549 - +0.7

Or see this region's nucleotide sequence