Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4807 and PP_4808 are separated by 16 nucleotides PP_4808 and PP_4809 overlap by 41 nucleotides PP_4809 and PP_4810 are separated by 43 nucleotides
PP_4807: PP_4807 - transpeptidase (penicillin-binding protein 2), at 5,470,015 to 5,471,904
_4807
PP_4808: PP_4808 - 23S rRNA m3psi1915 methyltransferase, at 5,471,921 to 5,472,439
_4808
PP_4809: PP_4809 - Ribosomal silencing factor RsfS, at 5,472,399 to 5,472,818
_4809
PP_4810: PP_4810 - Probable nicotinate-nucleotide adenylyltransferase, at 5,472,862 to 5,473,554
_4810
Position (kb)
5471
5472
5473 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5471.915 kb on - strand at 5471.915 kb on - strand at 5471.932 kb on - strand at 5471.965 kb on - strand at 5471.991 kb on - strand, within PP_4808 at 5472.322 kb on - strand, within PP_4808 at 5472.322 kb on - strand, within PP_4808 at 5472.401 kb on - strand at 5472.401 kb on - strand at 5472.697 kb on + strand, within PP_4809 at 5472.698 kb on - strand, within PP_4809 at 5472.698 kb on - strand, within PP_4809 at 5472.821 kb on - strand at 5472.821 kb on - strand at 5472.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs remove 5,471,915 - +1.5 5,471,915 - -1.3 5,471,932 - +1.4 5,471,965 - -2.2 5,471,991 - PP_4808 0.13 -2.8 5,472,322 - PP_4808 0.77 -3.5 5,472,322 - PP_4808 0.77 -2.4 5,472,401 - -4.5 5,472,401 - +1.8 5,472,697 + PP_4809 0.71 +2.2 5,472,698 - PP_4809 0.71 -2.0 5,472,698 - PP_4809 0.71 -0.4 5,472,821 - -3.3 5,472,821 - -1.7 5,472,821 - -3.1
Or see this region's nucleotide sequence