Strain Fitness in Pseudomonas putida KT2440 around PP_2530

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2528 and PP_2530 are separated by 220 nucleotidesPP_2530 and PP_2531 are separated by 18 nucleotides PP_2528: PP_2528 - O-acetylhomoserine (thiol)-lyase, at 2,874,605 to 2,875,882 _2528 PP_2530: PP_2530 - conserved protein of unknown function, at 2,876,103 to 2,876,360 _2530 PP_2531: PP_2531 - putative 5-nucleotidase, at 2,876,379 to 2,877,374 _2531 Position (kb) 2876 2877Strain fitness (log2 ratio) -3 -2 -1 0 1at 2875.158 kb on + strand, within PP_2528at 2875.269 kb on + strand, within PP_2528at 2875.269 kb on + strandat 2875.408 kb on + strand, within PP_2528at 2875.409 kb on - strandat 2875.442 kb on + strand, within PP_2528at 2875.443 kb on - strand, within PP_2528at 2875.552 kb on - strand, within PP_2528at 2875.728 kb on + strand, within PP_2528at 2875.728 kb on + strand, within PP_2528at 2875.806 kb on + strandat 2875.806 kb on + strandat 2875.807 kb on - strandat 2875.807 kb on - strandat 2875.807 kb on - strandat 2875.814 kb on + strandat 2876.001 kb on - strandat 2876.007 kb on - strandat 2876.007 kb on - strandat 2876.042 kb on + strandat 2876.043 kb on - strandat 2876.068 kb on + strandat 2876.069 kb on - strandat 2876.087 kb on - strandat 2876.262 kb on + strand, within PP_2530at 2876.263 kb on - strand, within PP_2530at 2876.380 kb on + strandat 2876.472 kb on + strandat 2876.473 kb on - strandat 2876.550 kb on + strand, within PP_2531at 2876.570 kb on + strand, within PP_2531at 2876.608 kb on - strand, within PP_2531at 2876.695 kb on + strand, within PP_2531at 2876.696 kb on - strand, within PP_2531at 2876.696 kb on - strand, within PP_2531at 2876.926 kb on + strand, within PP_2531at 2876.926 kb on + strand, within PP_2531at 2876.927 kb on - strand, within PP_2531at 2876.927 kb on - strand, within PP_2531at 2876.927 kb on - strand, within PP_2531at 2876.927 kb on - strand, within PP_2531at 2876.937 kb on - strand, within PP_2531at 2876.943 kb on + strand, within PP_2531at 2876.959 kb on + strand, within PP_2531at 2876.960 kb on - strand, within PP_2531at 2876.960 kb on - strand, within PP_2531at 2877.034 kb on + strand, within PP_2531at 2877.041 kb on - strand, within PP_2531at 2877.088 kb on + strand, within PP_2531at 2877.292 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
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2,875,158 + PP_2528 0.43 +0.3
2,875,269 + PP_2528 0.52 -0.6
2,875,269 + +0.3
2,875,408 + PP_2528 0.63 -0.3
2,875,409 - -1.2
2,875,442 + PP_2528 0.65 -0.1
2,875,443 - PP_2528 0.66 +0.6
2,875,552 - PP_2528 0.74 +0.6
2,875,728 + PP_2528 0.88 -1.2
2,875,728 + PP_2528 0.88 +0.9
2,875,806 + -0.2
2,875,806 + -0.2
2,875,807 - -0.1
2,875,807 - -0.8
2,875,807 - -0.8
2,875,814 + -0.1
2,876,001 - -1.4
2,876,007 - +0.8
2,876,007 - +0.9
2,876,042 + -0.2
2,876,043 - -0.6
2,876,068 + -2.0
2,876,069 - -0.4
2,876,087 - -0.7
2,876,262 + PP_2530 0.62 +0.7
2,876,263 - PP_2530 0.62 +1.0
2,876,380 + -0.9
2,876,472 + +0.8
2,876,473 - +0.7
2,876,550 + PP_2531 0.17 +0.3
2,876,570 + PP_2531 0.19 +0.3
2,876,608 - PP_2531 0.23 +0.9
2,876,695 + PP_2531 0.32 -0.1
2,876,696 - PP_2531 0.32 +0.9
2,876,696 - PP_2531 0.32 +0.7
2,876,926 + PP_2531 0.55 -0.9
2,876,926 + PP_2531 0.55 +0.1
2,876,927 - PP_2531 0.55 -0.6
2,876,927 - PP_2531 0.55 -2.3
2,876,927 - PP_2531 0.55 +0.3
2,876,927 - PP_2531 0.55 +0.1
2,876,937 - PP_2531 0.56 +0.6
2,876,943 + PP_2531 0.57 -0.6
2,876,959 + PP_2531 0.58 +0.2
2,876,960 - PP_2531 0.58 -3.2
2,876,960 - PP_2531 0.58 -0.8
2,877,034 + PP_2531 0.66 -1.5
2,877,041 - PP_2531 0.66 -0.1
2,877,088 + PP_2531 0.71 -2.4
2,877,292 + -0.7

Or see this region's nucleotide sequence