Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2528 and PP_2530 are separated by 220 nucleotides PP_2530 and PP_2531 are separated by 18 nucleotides
PP_2528: PP_2528 - O-acetylhomoserine (thiol)-lyase, at 2,874,605 to 2,875,882
_2528
PP_2530: PP_2530 - conserved protein of unknown function, at 2,876,103 to 2,876,360
_2530
PP_2531: PP_2531 - putative 5-nucleotidase, at 2,876,379 to 2,877,374
_2531
Position (kb)
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2875.158 kb on + strand, within PP_2528 at 2875.269 kb on + strand, within PP_2528 at 2875.269 kb on + strand at 2875.408 kb on + strand, within PP_2528 at 2875.409 kb on - strand at 2875.442 kb on + strand, within PP_2528 at 2875.443 kb on - strand, within PP_2528 at 2875.552 kb on - strand, within PP_2528 at 2875.728 kb on + strand, within PP_2528 at 2875.728 kb on + strand, within PP_2528 at 2875.806 kb on + strand at 2875.806 kb on + strand at 2875.807 kb on - strand at 2875.807 kb on - strand at 2875.807 kb on - strand at 2875.814 kb on + strand at 2876.001 kb on - strand at 2876.007 kb on - strand at 2876.007 kb on - strand at 2876.042 kb on + strand at 2876.043 kb on - strand at 2876.068 kb on + strand at 2876.069 kb on - strand at 2876.087 kb on - strand at 2876.262 kb on + strand, within PP_2530 at 2876.263 kb on - strand, within PP_2530 at 2876.380 kb on + strand at 2876.472 kb on + strand at 2876.473 kb on - strand at 2876.550 kb on + strand, within PP_2531 at 2876.570 kb on + strand, within PP_2531 at 2876.608 kb on - strand, within PP_2531 at 2876.695 kb on + strand, within PP_2531 at 2876.696 kb on - strand, within PP_2531 at 2876.696 kb on - strand, within PP_2531 at 2876.926 kb on + strand, within PP_2531 at 2876.926 kb on + strand, within PP_2531 at 2876.927 kb on - strand, within PP_2531 at 2876.927 kb on - strand, within PP_2531 at 2876.927 kb on - strand, within PP_2531 at 2876.927 kb on - strand, within PP_2531 at 2876.937 kb on - strand, within PP_2531 at 2876.943 kb on + strand, within PP_2531 at 2876.959 kb on + strand, within PP_2531 at 2876.960 kb on - strand, within PP_2531 at 2876.960 kb on - strand, within PP_2531 at 2877.034 kb on + strand, within PP_2531 at 2877.041 kb on - strand, within PP_2531 at 2877.088 kb on + strand, within PP_2531 at 2877.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs remove 2,875,158 + PP_2528 0.43 +0.3 2,875,269 + PP_2528 0.52 -0.6 2,875,269 + +0.3 2,875,408 + PP_2528 0.63 -0.3 2,875,409 - -1.2 2,875,442 + PP_2528 0.65 -0.1 2,875,443 - PP_2528 0.66 +0.6 2,875,552 - PP_2528 0.74 +0.6 2,875,728 + PP_2528 0.88 -1.2 2,875,728 + PP_2528 0.88 +0.9 2,875,806 + -0.2 2,875,806 + -0.2 2,875,807 - -0.1 2,875,807 - -0.8 2,875,807 - -0.8 2,875,814 + -0.1 2,876,001 - -1.4 2,876,007 - +0.8 2,876,007 - +0.9 2,876,042 + -0.2 2,876,043 - -0.6 2,876,068 + -2.0 2,876,069 - -0.4 2,876,087 - -0.7 2,876,262 + PP_2530 0.62 +0.7 2,876,263 - PP_2530 0.62 +1.0 2,876,380 + -0.9 2,876,472 + +0.8 2,876,473 - +0.7 2,876,550 + PP_2531 0.17 +0.3 2,876,570 + PP_2531 0.19 +0.3 2,876,608 - PP_2531 0.23 +0.9 2,876,695 + PP_2531 0.32 -0.1 2,876,696 - PP_2531 0.32 +0.9 2,876,696 - PP_2531 0.32 +0.7 2,876,926 + PP_2531 0.55 -0.9 2,876,926 + PP_2531 0.55 +0.1 2,876,927 - PP_2531 0.55 -0.6 2,876,927 - PP_2531 0.55 -2.3 2,876,927 - PP_2531 0.55 +0.3 2,876,927 - PP_2531 0.55 +0.1 2,876,937 - PP_2531 0.56 +0.6 2,876,943 + PP_2531 0.57 -0.6 2,876,959 + PP_2531 0.58 +0.2 2,876,960 - PP_2531 0.58 -3.2 2,876,960 - PP_2531 0.58 -0.8 2,877,034 + PP_2531 0.66 -1.5 2,877,041 - PP_2531 0.66 -0.1 2,877,088 + PP_2531 0.71 -2.4 2,877,292 + -0.7
Or see this region's nucleotide sequence