Strain Fitness in Pseudomonas putida KT2440 around PP_1214

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPP_1213 and PP_1214 are separated by 114 nucleotidesPP_1214 and PP_1215 are separated by 185 nucleotidesPP_1215 and PP_1216 are separated by 109 nucleotides PP_1213: PP_1213 - Aspartate--tRNA ligase, at 1,390,194 to 1,391,969 _1213 PP_1214: PP_1214 - putative transcriptional regulatory protein, at 1,392,084 to 1,392,830 _1214 PP_1215: PP_1215 - Crossover junction endodeoxyribonuclease RuvC, at 1,393,016 to 1,393,540 _1215 PP_1216: PP_1216 - Holliday junction ATP-dependent DNA helicase RuvA, at 1,393,650 to 1,394,267 _1216 Position (kb) 1392 1393Strain fitness (log2 ratio) -2 -1 0 1 2at 1392.624 kb on + strandat 1392.624 kb on + strand, within PP_1214at 1392.624 kb on + strand, within PP_1214at 1392.624 kb on + strand, within PP_1214at 1392.625 kb on - strand, within PP_1214at 1392.625 kb on - strand, within PP_1214at 1392.625 kb on - strand, within PP_1214at 1392.636 kb on - strandat 1392.636 kb on - strandat 1392.783 kb on + strandat 1392.784 kb on - strandat 1392.908 kb on - strandat 1392.913 kb on + strandat 1392.913 kb on + strandat 1392.914 kb on - strandat 1392.914 kb on - strandat 1392.916 kb on + strandat 1392.917 kb on - strandat 1392.941 kb on + strandat 1392.947 kb on + strandat 1392.948 kb on - strandat 1392.948 kb on - strandat 1392.959 kb on + strandat 1392.960 kb on - strandat 1393.015 kb on + strandat 1393.015 kb on + strandat 1393.033 kb on + strandat 1393.062 kb on - strandat 1393.072 kb on - strand, within PP_1215at 1393.097 kb on + strand, within PP_1215at 1393.098 kb on - strand, within PP_1215at 1393.538 kb on + strandat 1393.558 kb on - strandat 1393.577 kb on + strandat 1393.577 kb on + strandat 1393.577 kb on + strandat 1393.577 kb on + strandat 1393.578 kb on - strandat 1393.602 kb on + strandat 1393.602 kb on + strandat 1393.703 kb on + strandat 1393.704 kb on - strandat 1393.756 kb on - strand, within PP_1216at 1393.794 kb on - strand, within PP_1216

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
remove
1,392,624 + +1.6
1,392,624 + PP_1214 0.72 +1.6
1,392,624 + PP_1214 0.72 -0.2
1,392,624 + PP_1214 0.72 +0.0
1,392,625 - PP_1214 0.72 -0.8
1,392,625 - PP_1214 0.72 +2.5
1,392,625 - PP_1214 0.72 +0.5
1,392,636 - +1.8
1,392,636 - +1.2
1,392,783 + +0.8
1,392,784 - -2.7
1,392,908 - -0.6
1,392,913 + +1.0
1,392,913 + +0.1
1,392,914 - -2.0
1,392,914 - -0.4
1,392,916 + +0.9
1,392,917 - -0.4
1,392,941 + -1.9
1,392,947 + -0.6
1,392,948 - -1.9
1,392,948 - -2.5
1,392,959 + -0.2
1,392,960 - +0.6
1,393,015 + -0.5
1,393,015 + +0.4
1,393,033 + +1.8
1,393,062 - -0.1
1,393,072 - PP_1215 0.11 -0.1
1,393,097 + PP_1215 0.15 -1.5
1,393,098 - PP_1215 0.16 +1.0
1,393,538 + -0.7
1,393,558 - -0.4
1,393,577 + -0.8
1,393,577 + -1.1
1,393,577 + +0.0
1,393,577 + +0.3
1,393,578 - +0.9
1,393,602 + -0.8
1,393,602 + +0.8
1,393,703 + -1.2
1,393,704 - -0.5
1,393,756 - PP_1216 0.17 -1.5
1,393,794 - PP_1216 0.23 -0.2

Or see this region's nucleotide sequence