Strain Fitness in Pseudomonas putida KT2440 around PP_4356

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4354 and PP_4355 are separated by 21 nucleotidesPP_4355 and PP_4356 overlap by 1 nucleotidesPP_4356 and PP_4357 are separated by 12 nucleotidesPP_4357 and PP_4358 are separated by 29 nucleotides PP_4354: PP_4354 - flagellar export apparatus subunit, at 4,947,367 to 4,947,636 _4354 PP_4355: PP_4355 - flagellar export apparatus subunit, at 4,947,658 to 4,948,413 _4355 PP_4356: PP_4356 - Flagellar protein FliO, at 4,948,413 to 4,948,847 _4356 PP_4357: PP_4357 - C-ring protein of the flagellar basal body, at 4,948,860 to 4,949,345 _4357 PP_4358: PP_4358 - flagellar synthesis, switching and energizing component, at 4,949,375 to 4,950,343 _4358 Position (kb) 4948 4949Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4947.774 kb on + strand, within PP_4355at 4947.775 kb on - strand, within PP_4355at 4948.395 kb on + strandat 4948.396 kb on - strandat 4948.396 kb on - strandat 4948.396 kb on - strandat 4948.396 kb on - strandat 4948.518 kb on + strand, within PP_4356at 4948.518 kb on + strand, within PP_4356at 4948.547 kb on + strand, within PP_4356at 4948.547 kb on + strand, within PP_4356at 4948.877 kb on + strandat 4949.357 kb on + strandat 4949.357 kb on + strandat 4949.358 kb on - strandat 4949.358 kb on - strandat 4949.358 kb on - strandat 4949.811 kb on + strand, within PP_4358at 4949.812 kb on - strand, within PP_4358

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
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4,947,774 + PP_4355 0.15 -2.5
4,947,775 - PP_4355 0.15 -0.9
4,948,395 + -3.6
4,948,396 - -0.9
4,948,396 - -1.4
4,948,396 - -1.8
4,948,396 - -0.8
4,948,518 + PP_4356 0.24 -1.9
4,948,518 + PP_4356 0.24 -2.1
4,948,547 + PP_4356 0.31 -1.6
4,948,547 + PP_4356 0.31 -3.2
4,948,877 + -0.6
4,949,357 + -2.5
4,949,357 + -3.0
4,949,358 - +1.1
4,949,358 - +1.0
4,949,358 - -0.3
4,949,811 + PP_4358 0.45 -4.5
4,949,812 - PP_4358 0.45 -0.9

Or see this region's nucleotide sequence