Strain Fitness in Pseudomonas putida KT2440 around PP_2814

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2813 and PP_2814 are separated by 116 nucleotidesPP_2814 and PP_2815 are separated by 238 nucleotides PP_2813: PP_2813 - BNR domain protein, at 3,208,777 to 3,209,739 _2813 PP_2814: PP_2814 - conserved protein of unknown function, at 3,209,856 to 3,210,725 _2814 PP_2815: PP_2815 - conserved protein of unknown function, at 3,210,964 to 3,212,217 _2815 Position (kb) 3209 3210 3211Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3209.332 kb on + strand, within PP_2813at 3209.357 kb on + strand, within PP_2813at 3209.358 kb on - strand, within PP_2813at 3209.358 kb on - strand, within PP_2813at 3209.358 kb on - strand, within PP_2813at 3209.358 kb on - strand, within PP_2813at 3209.694 kb on - strandat 3209.699 kb on + strandat 3209.700 kb on - strandat 3209.870 kb on - strandat 3209.887 kb on + strandat 3209.887 kb on + strandat 3209.891 kb on + strandat 3209.891 kb on + strandat 3209.892 kb on - strandat 3209.892 kb on - strandat 3209.892 kb on - strandat 3210.002 kb on - strand, within PP_2814at 3210.058 kb on + strand, within PP_2814at 3210.061 kb on + strand, within PP_2814at 3210.062 kb on - strand, within PP_2814at 3210.062 kb on - strand, within PP_2814at 3210.305 kb on - strand, within PP_2814at 3210.404 kb on - strand, within PP_2814at 3210.860 kb on - strandat 3210.888 kb on + strandat 3210.888 kb on + strandat 3210.889 kb on - strandat 3210.889 kb on - strandat 3211.018 kb on - strandat 3211.018 kb on - strandat 3211.219 kb on + strandat 3211.220 kb on - strand, within PP_2815at 3211.486 kb on - strand, within PP_2815at 3211.491 kb on + strand, within PP_2815at 3211.565 kb on - strand, within PP_2815at 3211.565 kb on - strand, within PP_2815at 3211.565 kb on - strand, within PP_2815

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
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3,209,332 + PP_2813 0.58 +0.5
3,209,357 + PP_2813 0.60 +0.9
3,209,358 - PP_2813 0.60 -4.0
3,209,358 - PP_2813 0.60 +0.5
3,209,358 - PP_2813 0.60 +1.0
3,209,358 - PP_2813 0.60 -2.7
3,209,694 - +1.8
3,209,699 + -0.7
3,209,700 - -1.0
3,209,870 - -3.0
3,209,887 + -1.9
3,209,887 + +0.6
3,209,891 + -1.6
3,209,891 + -0.1
3,209,892 - -0.8
3,209,892 - -0.8
3,209,892 - +0.8
3,210,002 - PP_2814 0.17 -1.4
3,210,058 + PP_2814 0.23 -1.8
3,210,061 + PP_2814 0.24 -0.3
3,210,062 - PP_2814 0.24 -0.9
3,210,062 - PP_2814 0.24 -1.4
3,210,305 - PP_2814 0.52 -1.1
3,210,404 - PP_2814 0.63 +0.3
3,210,860 - -1.6
3,210,888 + -2.5
3,210,888 + +0.2
3,210,889 - +0.3
3,210,889 - -1.3
3,211,018 - -2.2
3,211,018 - +0.9
3,211,219 + -2.0
3,211,220 - PP_2815 0.20 -2.1
3,211,486 - PP_2815 0.42 -4.2
3,211,491 + PP_2815 0.42 -0.0
3,211,565 - PP_2815 0.48 -0.2
3,211,565 - PP_2815 0.48 -3.6
3,211,565 - PP_2815 0.48 +1.3

Or see this region's nucleotide sequence