Strain Fitness in Pseudomonas putida KT2440 around PP_2524

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2522 and PP_2524 are separated by 529 nucleotidesPP_2524 and PP_2525 are separated by 31 nucleotides PP_2522: PP_2522 - transposase, at 2,869,392 to 2,870,693 _2522 PP_2524: PP_2524 - HD domain protein, at 2,871,223 to 2,871,879 _2524 PP_2525: PP_2525 - conserved protein of unknown function, at 2,871,911 to 2,873,074 _2525 Position (kb) 2871 2872Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2870.774 kb on - strandat 2870.778 kb on + strandat 2870.779 kb on - strandat 2870.928 kb on + strandat 2870.929 kb on - strandat 2870.929 kb on - strandat 2870.930 kb on + strandat 2870.930 kb on + strandat 2871.056 kb on - strandat 2871.056 kb on - strandat 2871.079 kb on + strandat 2871.120 kb on + strandat 2871.120 kb on + strandat 2871.153 kb on + strandat 2871.215 kb on + strandat 2871.215 kb on + strandat 2871.216 kb on - strandat 2871.247 kb on + strandat 2871.277 kb on + strandat 2871.278 kb on - strandat 2871.321 kb on + strand, within PP_2524at 2871.440 kb on + strandat 2871.440 kb on + strand, within PP_2524at 2871.441 kb on - strand, within PP_2524at 2871.527 kb on + strand, within PP_2524at 2871.528 kb on - strand, within PP_2524at 2871.554 kb on - strand, within PP_2524at 2871.652 kb on + strand, within PP_2524at 2871.683 kb on - strand, within PP_2524at 2871.683 kb on - strand, within PP_2524at 2871.783 kb on + strand, within PP_2524at 2871.784 kb on - strand, within PP_2524at 2871.784 kb on - strand, within PP_2524at 2871.894 kb on + strandat 2871.895 kb on - strandat 2871.992 kb on + strandat 2871.993 kb on - strandat 2872.050 kb on - strand, within PP_2525at 2872.265 kb on + strand, within PP_2525at 2872.266 kb on - strand, within PP_2525at 2872.695 kb on - strand, within PP_2525

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
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2,870,774 - -2.0
2,870,778 + +0.8
2,870,779 - -0.5
2,870,928 + -1.0
2,870,929 - -3.6
2,870,929 - -3.3
2,870,930 + +0.6
2,870,930 + -0.4
2,871,056 - +1.7
2,871,056 - +2.1
2,871,079 + +0.3
2,871,120 + +1.3
2,871,120 + -0.3
2,871,153 + -0.5
2,871,215 + +0.7
2,871,215 + -1.6
2,871,216 - +0.4
2,871,247 + -3.9
2,871,277 + -1.4
2,871,278 - +0.2
2,871,321 + PP_2524 0.15 -0.2
2,871,440 + -0.1
2,871,440 + PP_2524 0.33 +1.5
2,871,441 - PP_2524 0.33 -0.5
2,871,527 + PP_2524 0.46 -2.3
2,871,528 - PP_2524 0.46 -0.6
2,871,554 - PP_2524 0.50 -0.6
2,871,652 + PP_2524 0.65 -0.2
2,871,683 - PP_2524 0.70 +0.4
2,871,683 - PP_2524 0.70 -0.8
2,871,783 + PP_2524 0.85 -3.4
2,871,784 - PP_2524 0.85 -2.2
2,871,784 - PP_2524 0.85 -0.7
2,871,894 + +0.2
2,871,895 - -2.4
2,871,992 + +2.0
2,871,993 - +0.9
2,872,050 - PP_2525 0.12 +0.2
2,872,265 + PP_2525 0.30 -0.5
2,872,266 - PP_2525 0.30 +0.3
2,872,695 - PP_2525 0.67 +0.7

Or see this region's nucleotide sequence