Strain Fitness in Pseudomonas putida KT2440 around PP_1749

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1748 and PP_1749 are separated by 169 nucleotidesPP_1749 and PP_1750 are separated by 3 nucleotides PP_1748: PP_1748 - conserved protein of unknown function, at 1,946,555 to 1,947,739 _1748 PP_1749: PP_1749 - GNAT family acetyltransferase, at 1,947,909 to 1,949,654 _1749 PP_1750: PP_1750 - asparagine synthetase, at 1,949,658 to 1,951,445 _1750 Position (kb) 1947 1948 1949 1950Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1946.919 kb on + strand, within PP_1748at 1946.919 kb on + strand, within PP_1748at 1946.926 kb on - strand, within PP_1748at 1947.153 kb on - strand, within PP_1748at 1947.283 kb on - strand, within PP_1748at 1947.633 kb on + strandat 1947.634 kb on - strandat 1947.825 kb on + strandat 1947.848 kb on + strandat 1948.019 kb on - strandat 1948.214 kb on - strand, within PP_1749at 1948.337 kb on + strand, within PP_1749at 1948.829 kb on - strand, within PP_1749at 1948.936 kb on + strand, within PP_1749at 1948.985 kb on - strand, within PP_1749at 1948.985 kb on - strand, within PP_1749at 1949.183 kb on - strand, within PP_1749at 1949.210 kb on + strand, within PP_1749at 1949.252 kb on - strand, within PP_1749at 1949.316 kb on + strandat 1949.425 kb on + strand, within PP_1749at 1949.426 kb on - strand, within PP_1749at 1949.426 kb on - strand, within PP_1749at 1949.426 kb on - strand, within PP_1749at 1949.506 kb on - strandat 1949.506 kb on - strandat 1949.632 kb on + strandat 1949.872 kb on + strand, within PP_1750at 1949.872 kb on + strand, within PP_1750at 1949.873 kb on - strand, within PP_1750at 1949.873 kb on - strand, within PP_1750at 1949.999 kb on - strand, within PP_1750at 1950.208 kb on + strand, within PP_1750at 1950.254 kb on - strand, within PP_1750at 1950.254 kb on - strand, within PP_1750at 1950.254 kb on - strand, within PP_1750at 1950.254 kb on - strand, within PP_1750at 1950.330 kb on + strand, within PP_1750at 1950.331 kb on - strand, within PP_1750at 1950.370 kb on + strand, within PP_1750at 1950.370 kb on + strand, within PP_1750at 1950.370 kb on + strand, within PP_1750at 1950.371 kb on - strand, within PP_1750at 1950.371 kb on - strand, within PP_1750at 1950.371 kb on - strand, within PP_1750at 1950.484 kb on + strand, within PP_1750at 1950.484 kb on + strand, within PP_1750at 1950.484 kb on + strand, within PP_1750

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
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1,946,919 + PP_1748 0.31 +0.5
1,946,919 + PP_1748 0.31 -0.4
1,946,926 - PP_1748 0.31 +1.0
1,947,153 - PP_1748 0.50 +0.5
1,947,283 - PP_1748 0.61 -0.9
1,947,633 + -0.8
1,947,634 - +0.3
1,947,825 + +0.1
1,947,848 + +0.9
1,948,019 - +0.4
1,948,214 - PP_1749 0.17 -2.0
1,948,337 + PP_1749 0.25 -0.1
1,948,829 - PP_1749 0.53 +1.1
1,948,936 + PP_1749 0.59 +1.9
1,948,985 - PP_1749 0.62 +0.7
1,948,985 - PP_1749 0.62 +1.1
1,949,183 - PP_1749 0.73 +1.0
1,949,210 + PP_1749 0.75 +0.2
1,949,252 - PP_1749 0.77 -1.7
1,949,316 + -0.0
1,949,425 + PP_1749 0.87 +0.6
1,949,426 - PP_1749 0.87 +0.9
1,949,426 - PP_1749 0.87 +1.2
1,949,426 - PP_1749 0.87 +2.1
1,949,506 - -0.5
1,949,506 - +0.5
1,949,632 + +0.1
1,949,872 + PP_1750 0.12 -0.2
1,949,872 + PP_1750 0.12 +0.7
1,949,873 - PP_1750 0.12 -0.0
1,949,873 - PP_1750 0.12 -1.3
1,949,999 - PP_1750 0.19 -3.1
1,950,208 + PP_1750 0.31 -1.0
1,950,254 - PP_1750 0.33 +0.4
1,950,254 - PP_1750 0.33 +0.3
1,950,254 - PP_1750 0.33 -0.6
1,950,254 - PP_1750 0.33 +1.0
1,950,330 + PP_1750 0.38 +0.8
1,950,331 - PP_1750 0.38 +0.1
1,950,370 + PP_1750 0.40 +0.8
1,950,370 + PP_1750 0.40 -1.9
1,950,370 + PP_1750 0.40 -1.0
1,950,371 - PP_1750 0.40 +0.0
1,950,371 - PP_1750 0.40 -3.2
1,950,371 - PP_1750 0.40 +0.3
1,950,484 + PP_1750 0.46 -0.4
1,950,484 + PP_1750 0.46 -3.1
1,950,484 + PP_1750 0.46 +1.0

Or see this region's nucleotide sequence