Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4348 and PP_4349 are separated by 33 nucleotides PP_4349 and PP_4350 are separated by 10 nucleotides PP_4350 and PP_5673 overlap by 4 nucleotides
PP_4348: PP_4348 - putative Cystathionine beta-lyase, at 4,940,549 to 4,941,643
_4348
PP_4349: PP_4349 - conserved protein of unknown function, at 4,941,677 to 4,942,555
_4349
PP_4350: PP_4350 - Aminotransferase, class V, NifS/IscS family, at 4,942,566 to 4,943,729
_4350
PP_5673: PP_5673 - putative D-alanine--D-alanine ligase, at 4,943,726 to 4,944,640
_5673
Position (kb) 4942
4943
4944 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 4941.712 kb on + strand at 4941.852 kb on + strand, within PP_4349 at 4941.853 kb on - strand, within PP_4349 at 4941.853 kb on - strand, within PP_4349 at 4941.925 kb on + strand, within PP_4349 at 4941.927 kb on + strand, within PP_4349 at 4941.946 kb on + strand, within PP_4349 at 4942.031 kb on + strand, within PP_4349 at 4942.033 kb on + strand, within PP_4349 at 4942.033 kb on + strand, within PP_4349 at 4942.142 kb on - strand, within PP_4349 at 4942.291 kb on - strand, within PP_4349 at 4942.468 kb on - strand at 4942.544 kb on + strand at 4942.587 kb on + strand at 4942.592 kb on - strand at 4942.592 kb on - strand at 4942.592 kb on - strand at 4942.748 kb on - strand, within PP_4350 at 4942.800 kb on - strand, within PP_4350 at 4942.848 kb on - strand, within PP_4350 at 4942.909 kb on + strand, within PP_4350 at 4942.963 kb on + strand, within PP_4350 at 4943.116 kb on + strand, within PP_4350 at 4943.202 kb on + strand, within PP_4350 at 4943.203 kb on - strand, within PP_4350 at 4943.499 kb on - strand, within PP_4350 at 4943.501 kb on + strand, within PP_4350 at 4943.565 kb on + strand, within PP_4350 at 4943.565 kb on + strand, within PP_4350 at 4943.951 kb on + strand, within PP_5673 at 4943.956 kb on - strand, within PP_5673 at 4943.975 kb on + strand, within PP_5673 at 4944.035 kb on - strand, within PP_5673 at 4944.075 kb on - strand, within PP_5673 at 4944.164 kb on - strand, within PP_5673 at 4944.265 kb on - strand, within PP_5673 at 4944.350 kb on + strand, within PP_5673 at 4944.399 kb on + strand, within PP_5673 at 4944.399 kb on + strand, within PP_5673 at 4944.400 kb on - strand, within PP_5673 at 4944.472 kb on + strand, within PP_5673 at 4944.507 kb on - strand, within PP_5673 at 4944.600 kb on + strand at 4944.604 kb on + strand at 4944.703 kb on + strand at 4944.709 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs remove 4,941,712 + +0.6 4,941,852 + PP_4349 0.20 +2.5 4,941,853 - PP_4349 0.20 -1.0 4,941,853 - PP_4349 0.20 -0.6 4,941,925 + PP_4349 0.28 -0.2 4,941,927 + PP_4349 0.28 -0.2 4,941,946 + PP_4349 0.31 -0.1 4,942,031 + PP_4349 0.40 +1.2 4,942,033 + PP_4349 0.41 +0.2 4,942,033 + PP_4349 0.41 -0.3 4,942,142 - PP_4349 0.53 -0.8 4,942,291 - PP_4349 0.70 -3.3 4,942,468 - +1.3 4,942,544 + -0.4 4,942,587 + -0.4 4,942,592 - +0.2 4,942,592 - +0.9 4,942,592 - -0.5 4,942,748 - PP_4350 0.16 +0.0 4,942,800 - PP_4350 0.20 -2.8 4,942,848 - PP_4350 0.24 -4.8 4,942,909 + PP_4350 0.29 +0.7 4,942,963 + PP_4350 0.34 +1.1 4,943,116 + PP_4350 0.47 -4.3 4,943,202 + PP_4350 0.55 -0.6 4,943,203 - PP_4350 0.55 -0.8 4,943,499 - PP_4350 0.80 -1.5 4,943,501 + PP_4350 0.80 -2.0 4,943,565 + PP_4350 0.86 -3.8 4,943,565 + PP_4350 0.86 -2.8 4,943,951 + PP_5673 0.25 +0.5 4,943,956 - PP_5673 0.25 +0.3 4,943,975 + PP_5673 0.27 +0.3 4,944,035 - PP_5673 0.34 -0.5 4,944,075 - PP_5673 0.38 +0.3 4,944,164 - PP_5673 0.48 +0.5 4,944,265 - PP_5673 0.59 +1.4 4,944,350 + PP_5673 0.68 -0.1 4,944,399 + PP_5673 0.74 +0.7 4,944,399 + PP_5673 0.74 -0.6 4,944,400 - PP_5673 0.74 -0.1 4,944,472 + PP_5673 0.82 +0.9 4,944,507 - PP_5673 0.85 -3.9 4,944,600 + +1.0 4,944,604 + -2.3 4,944,703 + -1.9 4,944,709 - +0.6
Or see this region's nucleotide sequence