Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0047 and PP_0048 are separated by 117 nucleotides PP_0048 and PP_5423 are separated by 101 nucleotides PP_5423 and PP_0049 are separated by 189 nucleotides
PP_0047: PP_0047 - response regulator, at 54,227 to 54,901
_0047
PP_0048: PP_0048 - conserved protein of unknown function, at 55,019 to 55,342
_0048
PP_5423: PP_5423 - conserved membrane protein of unknown function, at 55,444 to 55,971
_5423
PP_0049: PP_0049 - conserved protein of unknown function, at 56,161 to 57,627
_0049
Position (kb)
55
56 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 54.107 kb on - strand at 54.306 kb on + strand, within PP_0047 at 54.306 kb on + strand, within PP_0047 at 54.307 kb on - strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.382 kb on - strand, within PP_0047 at 54.449 kb on - strand, within PP_0047 at 54.600 kb on + strand, within PP_0047 at 54.601 kb on - strand, within PP_0047 at 54.689 kb on + strand, within PP_0047 at 54.699 kb on + strand, within PP_0047 at 54.718 kb on + strand, within PP_0047 at 54.799 kb on - strand, within PP_0047 at 54.831 kb on + strand, within PP_0047 at 54.832 kb on - strand, within PP_0047 at 54.832 kb on - strand, within PP_0047 at 54.862 kb on + strand at 54.863 kb on - strand at 54.900 kb on - strand at 55.016 kb on + strand at 55.017 kb on - strand at 55.025 kb on + strand at 55.025 kb on + strand at 55.027 kb on + strand at 55.028 kb on - strand at 55.028 kb on - strand at 55.214 kb on + strand, within PP_0048 at 55.377 kb on - strand at 55.465 kb on - strand at 55.537 kb on + strand, within PP_5423 at 55.867 kb on - strand, within PP_5423 at 55.877 kb on - strand, within PP_5423 at 55.981 kb on + strand at 55.981 kb on + strand at 55.982 kb on - strand at 55.993 kb on + strand at 56.007 kb on - strand at 56.126 kb on - strand at 56.126 kb on - strand at 56.214 kb on + strand at 56.251 kb on + strand at 56.251 kb on + strand at 56.258 kb on - strand at 56.293 kb on + strand at 56.320 kb on + strand, within PP_0049 at 56.331 kb on + strand, within PP_0049
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs remove 54,107 - -2.8 54,306 + PP_0047 0.12 +0.5 54,306 + PP_0047 0.12 -0.2 54,307 - PP_0047 0.12 +0.7 54,381 + PP_0047 0.23 +0.3 54,381 + PP_0047 0.23 -0.0 54,381 + PP_0047 0.23 -0.1 54,382 - PP_0047 0.23 +0.7 54,449 - PP_0047 0.33 -1.0 54,600 + PP_0047 0.55 +0.5 54,601 - PP_0047 0.55 +0.0 54,689 + PP_0047 0.68 +0.3 54,699 + PP_0047 0.70 +2.0 54,718 + PP_0047 0.73 +0.7 54,799 - PP_0047 0.85 -1.6 54,831 + PP_0047 0.89 -1.2 54,832 - PP_0047 0.90 +0.1 54,832 - PP_0047 0.90 +0.3 54,862 + -0.7 54,863 - +0.5 54,900 - +0.9 55,016 + -0.4 55,017 - -0.4 55,025 + -0.0 55,025 + -0.1 55,027 + -1.0 55,028 - -1.5 55,028 - -0.3 55,214 + PP_0048 0.60 +0.5 55,377 - +0.4 55,465 - -0.5 55,537 + PP_5423 0.18 +0.7 55,867 - PP_5423 0.80 +0.5 55,877 - PP_5423 0.82 -1.1 55,981 + +0.2 55,981 + -0.5 55,982 - +0.6 55,993 + -1.5 56,007 - +0.2 56,126 - -0.2 56,126 - +0.1 56,214 + -0.8 56,251 + -0.2 56,251 + +1.4 56,258 - -0.3 56,293 + -0.7 56,320 + PP_0049 0.11 -0.0 56,331 + PP_0049 0.12 -1.5
Or see this region's nucleotide sequence