Experiment: R2A control with 0.2% methanol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18065 and PS417_18070 overlap by 4 nucleotides PS417_18070 and PS417_18075 are separated by 7 nucleotides PS417_18075 and PS417_18080 are separated by 13 nucleotides PS417_18080 and PS417_18085 overlap by 4 nucleotides
PS417_18065: PS417_18065 - general secretion pathway protein GspI, at 3,935,773 to 3,936,105
_18065
PS417_18070: PS417_18070 - general secretion pathway protein GspH, at 3,936,102 to 3,936,527
_18070
PS417_18075: PS417_18075 - general secretion pathway protein GspG, at 3,936,535 to 3,936,924
_18075
PS417_18080: PS417_18080 - general secretion pathway protein GspF, at 3,936,938 to 3,938,134
_18080
PS417_18085: PS417_18085 - general secretion pathway protein GspE, at 3,938,131 to 3,939,822
_18085
Position (kb)
3936
3937
3938
3939 Strain fitness (log2 ratio)
-2
-1
0
1 at 3936.214 kb on + strand, within PS417_18070 at 3936.215 kb on - strand at 3936.510 kb on - strand at 3936.596 kb on + strand, within PS417_18075 at 3936.597 kb on - strand, within PS417_18075 at 3936.686 kb on + strand, within PS417_18075 at 3936.687 kb on - strand, within PS417_18075 at 3936.715 kb on + strand, within PS417_18075 at 3936.716 kb on - strand, within PS417_18075 at 3936.716 kb on - strand, within PS417_18075 at 3936.801 kb on - strand, within PS417_18075 at 3936.801 kb on - strand, within PS417_18075 at 3936.941 kb on + strand at 3936.941 kb on + strand at 3936.942 kb on - strand at 3936.943 kb on + strand at 3936.944 kb on - strand at 3936.944 kb on - strand at 3937.399 kb on - strand, within PS417_18080 at 3937.581 kb on + strand, within PS417_18080 at 3937.582 kb on - strand, within PS417_18080 at 3937.582 kb on - strand, within PS417_18080 at 3937.582 kb on - strand, within PS417_18080 at 3937.610 kb on + strand, within PS417_18080 at 3937.635 kb on + strand, within PS417_18080 at 3937.687 kb on - strand, within PS417_18080 at 3937.755 kb on + strand, within PS417_18080 at 3937.755 kb on + strand, within PS417_18080 at 3938.161 kb on + strand at 3938.161 kb on + strand at 3938.162 kb on - strand at 3938.162 kb on - strand at 3938.179 kb on - strand at 3938.181 kb on + strand at 3938.213 kb on + strand at 3938.339 kb on + strand, within PS417_18085 at 3938.501 kb on + strand, within PS417_18085 at 3938.501 kb on + strand, within PS417_18085 at 3938.502 kb on - strand, within PS417_18085 at 3938.810 kb on + strand, within PS417_18085 at 3938.810 kb on + strand, within PS417_18085 at 3938.811 kb on - strand, within PS417_18085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control with 0.2% methanol remove 3,936,214 + PS417_18070 0.26 -0.4 3,936,215 - +1.6 3,936,510 - -0.9 3,936,596 + PS417_18075 0.16 -0.4 3,936,597 - PS417_18075 0.16 -0.1 3,936,686 + PS417_18075 0.39 -1.7 3,936,687 - PS417_18075 0.39 +0.4 3,936,715 + PS417_18075 0.46 +0.4 3,936,716 - PS417_18075 0.46 -2.5 3,936,716 - PS417_18075 0.46 -0.8 3,936,801 - PS417_18075 0.68 -0.1 3,936,801 - PS417_18075 0.68 -0.1 3,936,941 + +1.2 3,936,941 + +0.1 3,936,942 - -0.0 3,936,943 + +0.1 3,936,944 - -0.3 3,936,944 - -0.4 3,937,399 - PS417_18080 0.39 -2.0 3,937,581 + PS417_18080 0.54 -1.5 3,937,582 - PS417_18080 0.54 +0.5 3,937,582 - PS417_18080 0.54 -0.7 3,937,582 - PS417_18080 0.54 +0.4 3,937,610 + PS417_18080 0.56 -1.8 3,937,635 + PS417_18080 0.58 -0.1 3,937,687 - PS417_18080 0.63 -0.7 3,937,755 + PS417_18080 0.68 -0.9 3,937,755 + PS417_18080 0.68 -1.0 3,938,161 + +0.6 3,938,161 + +0.4 3,938,162 - -0.3 3,938,162 - +0.1 3,938,179 - -0.8 3,938,181 + +0.6 3,938,213 + +0.0 3,938,339 + PS417_18085 0.12 -0.4 3,938,501 + PS417_18085 0.22 -0.3 3,938,501 + PS417_18085 0.22 +0.2 3,938,502 - PS417_18085 0.22 +1.6 3,938,810 + PS417_18085 0.40 +0.0 3,938,810 + PS417_18085 0.40 -0.6 3,938,811 - PS417_18085 0.40 -0.3
Or see this region's nucleotide sequence