Strain Fitness in Pseudomonas simiae WCS417 around PS417_16030

Experiment: R2A control with 0.2% methanol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_16020 and PS417_16025 are separated by 217 nucleotidesPS417_16025 and PS417_16030 overlap by 4 nucleotidesPS417_16030 and PS417_16035 are separated by 406 nucleotides PS417_16020: PS417_16020 - LysR family transcriptional regulator, at 3,511,436 to 3,512,341 _16020 PS417_16025: PS417_16025 - cysteine dioxygenase, at 3,512,559 to 3,513,170 _16025 PS417_16030: PS417_16030 - sulfurtransferase, at 3,513,167 to 3,514,750 _16030 PS417_16035: PS417_16035 - ABC transporter substrate-binding protein, at 3,515,157 to 3,516,131 _16035 Position (kb) 3513 3514 3515Strain fitness (log2 ratio) -2 -1 0 1at 3512.355 kb on - strandat 3512.386 kb on + strandat 3512.386 kb on + strandat 3512.386 kb on + strandat 3512.387 kb on - strandat 3512.436 kb on + strandat 3512.457 kb on - strandat 3512.589 kb on - strandat 3512.589 kb on - strandat 3512.600 kb on - strandat 3513.326 kb on + strand, within PS417_16030at 3513.326 kb on + strand, within PS417_16030at 3513.326 kb on + strand, within PS417_16030at 3513.327 kb on - strand, within PS417_16030at 3513.327 kb on - strand, within PS417_16030at 3513.570 kb on + strand, within PS417_16030at 3513.571 kb on - strand, within PS417_16030at 3513.681 kb on + strand, within PS417_16030at 3514.010 kb on + strand, within PS417_16030at 3514.010 kb on + strand, within PS417_16030at 3514.011 kb on - strand, within PS417_16030at 3514.023 kb on + strand, within PS417_16030at 3514.023 kb on + strand, within PS417_16030at 3514.023 kb on + strand, within PS417_16030at 3514.023 kb on + strand, within PS417_16030at 3514.208 kb on + strand, within PS417_16030at 3514.208 kb on + strand, within PS417_16030at 3514.370 kb on + strand, within PS417_16030at 3514.370 kb on + strand, within PS417_16030at 3514.371 kb on - strand, within PS417_16030at 3514.457 kb on + strand, within PS417_16030at 3514.457 kb on + strandat 3514.457 kb on + strand, within PS417_16030at 3514.458 kb on - strand, within PS417_16030at 3514.458 kb on - strand, within PS417_16030at 3514.843 kb on + strandat 3515.418 kb on + strand, within PS417_16035at 3515.419 kb on - strand, within PS417_16035at 3515.419 kb on - strand, within PS417_16035

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A control with 0.2% methanol
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3,512,355 - -0.3
3,512,386 + +0.3
3,512,386 + -0.5
3,512,386 + +0.5
3,512,387 - +1.4
3,512,436 + -0.2
3,512,457 - -0.8
3,512,589 - -0.2
3,512,589 - +1.1
3,512,600 - -1.1
3,513,326 + PS417_16030 0.10 -0.8
3,513,326 + PS417_16030 0.10 -0.9
3,513,326 + PS417_16030 0.10 -0.4
3,513,327 - PS417_16030 0.10 -0.7
3,513,327 - PS417_16030 0.10 -0.5
3,513,570 + PS417_16030 0.25 -1.9
3,513,571 - PS417_16030 0.26 -0.8
3,513,681 + PS417_16030 0.32 -0.1
3,514,010 + PS417_16030 0.53 -0.0
3,514,010 + PS417_16030 0.53 -2.0
3,514,011 - PS417_16030 0.53 -0.4
3,514,023 + PS417_16030 0.54 -0.1
3,514,023 + PS417_16030 0.54 -1.1
3,514,023 + PS417_16030 0.54 -0.2
3,514,023 + PS417_16030 0.54 -0.1
3,514,208 + PS417_16030 0.66 +0.0
3,514,208 + PS417_16030 0.66 -0.1
3,514,370 + PS417_16030 0.76 -1.0
3,514,370 + PS417_16030 0.76 -0.7
3,514,371 - PS417_16030 0.76 +0.8
3,514,457 + PS417_16030 0.81 -0.5
3,514,457 + +1.1
3,514,457 + PS417_16030 0.81 -0.6
3,514,458 - PS417_16030 0.82 -0.6
3,514,458 - PS417_16030 0.82 -0.6
3,514,843 + +0.3
3,515,418 + PS417_16035 0.27 -0.2
3,515,419 - PS417_16035 0.27 -1.3
3,515,419 - PS417_16035 0.27 -0.6

Or see this region's nucleotide sequence