Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02445 and PS417_02450 are separated by 108 nucleotides PS417_02450 and PS417_02455 are separated by 194 nucleotides PS417_02455 and PS417_02460 overlap by 4 nucleotides
PS417_02445: PS417_02445 - GTPase RsgA, at 560,128 to 561,159
_02445
PS417_02450: PS417_02450 - oligoribonuclease, at 561,268 to 561,810
_02450
PS417_02455: PS417_02455 - putative transporter, required for glycine utilization (from data) , at 562,005 to 562,619
_02455
PS417_02460: PS417_02460 - epoxyqueuosine reductase, at 562,616 to 563,680
_02460
Position (kb)
562
563 Strain fitness (log2 ratio)
-2
-1
0
1 at 561.192 kb on + strand at 561.209 kb on + strand at 561.240 kb on + strand at 561.241 kb on - strand at 561.241 kb on - strand at 561.241 kb on - strand at 562.020 kb on + strand at 562.020 kb on + strand at 562.447 kb on - strand, within PS417_02455 at 562.480 kb on - strand, within PS417_02455 at 562.480 kb on - strand, within PS417_02455 at 562.482 kb on - strand, within PS417_02455 at 562.482 kb on - strand, within PS417_02455 at 562.494 kb on + strand, within PS417_02455 at 562.600 kb on - strand at 562.618 kb on - strand at 562.828 kb on + strand, within PS417_02460 at 562.828 kb on + strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 563.041 kb on - strand, within PS417_02460 at 563.167 kb on - strand, within PS417_02460 at 563.190 kb on - strand, within PS417_02460 at 563.379 kb on + strand, within PS417_02460 at 563.380 kb on - strand, within PS417_02460 at 563.402 kb on + strand, within PS417_02460 at 563.434 kb on - strand, within PS417_02460 at 563.434 kb on - strand, within PS417_02460 at 563.517 kb on + strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 561,192 + -0.3 561,209 + -0.1 561,240 + +0.1 561,241 - -0.1 561,241 - +0.6 561,241 - -0.1 562,020 + -0.9 562,020 + -2.4 562,447 - PS417_02455 0.72 +0.4 562,480 - PS417_02455 0.77 -0.5 562,480 - PS417_02455 0.77 +0.5 562,482 - PS417_02455 0.78 -0.1 562,482 - PS417_02455 0.78 +0.1 562,494 + PS417_02455 0.80 +0.5 562,600 - +0.1 562,618 - -0.2 562,828 + PS417_02460 0.20 -1.9 562,828 + PS417_02460 0.20 +0.0 562,829 - PS417_02460 0.20 -0.2 562,829 - PS417_02460 0.20 -1.0 562,829 - PS417_02460 0.20 +0.6 563,041 - PS417_02460 0.40 -0.0 563,167 - PS417_02460 0.52 -1.4 563,190 - PS417_02460 0.54 -0.1 563,379 + PS417_02460 0.72 -0.2 563,380 - PS417_02460 0.72 +0.9 563,402 + PS417_02460 0.74 -0.2 563,434 - PS417_02460 0.77 +0.7 563,434 - PS417_02460 0.77 +0.9 563,517 + PS417_02460 0.85 -0.8 563,518 - PS417_02460 0.85 -0.2 563,518 - PS417_02460 0.85 -0.3 563,518 - PS417_02460 0.85 +0.6
Or see this region's nucleotide sequence