Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02200 and PS417_02205 are separated by 138 nucleotides PS417_02205 and PS417_02210 are separated by 72 nucleotides
PS417_02200: PS417_02200 - lactate dehydrogenase, at 495,965 to 496,801
_02200
PS417_02205: PS417_02205 - peptide methionine sulfoxide reductase, at 496,940 to 497,587
_02205
PS417_02210: PS417_02210 - diguanylate cyclase, at 497,660 to 500,353
_02210
Position (kb)
496
497
498 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 496.810 kb on + strand at 496.811 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.870 kb on + strand at 496.873 kb on + strand at 496.874 kb on - strand at 496.941 kb on + strand at 496.981 kb on - strand at 497.005 kb on - strand, within PS417_02205 at 497.005 kb on - strand, within PS417_02205 at 497.016 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.101 kb on - strand, within PS417_02205 at 497.151 kb on + strand, within PS417_02205 at 497.152 kb on - strand, within PS417_02205 at 497.166 kb on + strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.322 kb on + strand, within PS417_02205 at 497.323 kb on - strand, within PS417_02205 at 497.822 kb on - strand at 497.941 kb on - strand, within PS417_02210 at 497.941 kb on - strand, within PS417_02210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 496,810 + -0.5 496,811 - +0.5 496,863 - +1.3 496,863 - +0.4 496,863 - +0.1 496,863 - -1.4 496,870 + +0.0 496,873 + -0.5 496,874 - +0.4 496,941 + +0.4 496,981 - +0.9 497,005 - PS417_02205 0.10 -0.1 497,005 - PS417_02205 0.10 -0.7 497,016 + PS417_02205 0.12 -0.1 497,031 + PS417_02205 0.14 -1.0 497,031 + PS417_02205 0.14 -1.3 497,101 - PS417_02205 0.25 +0.0 497,151 + PS417_02205 0.33 -0.9 497,152 - PS417_02205 0.33 -0.6 497,166 + PS417_02205 0.35 -0.4 497,269 - PS417_02205 0.51 -1.4 497,269 - PS417_02205 0.51 -0.8 497,269 - PS417_02205 0.51 -0.2 497,269 - PS417_02205 0.51 -1.9 497,322 + PS417_02205 0.59 -0.3 497,323 - PS417_02205 0.59 -1.4 497,822 - +0.1 497,941 - PS417_02210 0.10 -1.3 497,941 - PS417_02210 0.10 +2.0
Or see this region's nucleotide sequence