Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17840 and PS417_17845 are separated by 49 nucleotides PS417_17845 and PS417_17850 are separated by 15 nucleotides PS417_17850 and PS417_17855 are separated by 63 nucleotides
PS417_17840: PS417_17840 - betaine-aldehyde dehydrogenase, at 3,895,311 to 3,896,771
_17840
PS417_17845: PS417_17845 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 3,896,821 to 3,897,678
_17845
PS417_17850: PS417_17850 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 3,897,694 to 3,898,107
_17850
PS417_17855: PS417_17855 - major facilitator transporter, at 3,898,171 to 3,899,475
_17855
Position (kb)
3896
3897
3898 Strain fitness (log2 ratio)
-1
0
1 at 3896.542 kb on - strand, within PS417_17840 at 3896.792 kb on - strand at 3896.805 kb on - strand at 3896.858 kb on + strand at 3896.870 kb on - strand at 3897.046 kb on + strand, within PS417_17845 at 3897.088 kb on + strand, within PS417_17845 at 3897.217 kb on + strand, within PS417_17845 at 3897.217 kb on + strand, within PS417_17845 at 3897.218 kb on - strand, within PS417_17845 at 3898.271 kb on - strand at 3898.271 kb on - strand at 3898.354 kb on + strand, within PS417_17855 at 3898.519 kb on + strand, within PS417_17855 at 3898.520 kb on - strand, within PS417_17855 at 3898.520 kb on - strand, within PS417_17855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,896,542 - PS417_17840 0.84 -0.1 3,896,792 - -0.6 3,896,805 - +0.6 3,896,858 + +0.1 3,896,870 - -0.3 3,897,046 + PS417_17845 0.26 -0.1 3,897,088 + PS417_17845 0.31 +1.2 3,897,217 + PS417_17845 0.46 -1.1 3,897,217 + PS417_17845 0.46 +0.5 3,897,218 - PS417_17845 0.46 -0.2 3,898,271 - -0.5 3,898,271 - -0.2 3,898,354 + PS417_17855 0.14 +1.7 3,898,519 + PS417_17855 0.27 +0.3 3,898,520 - PS417_17855 0.27 -0.2 3,898,520 - PS417_17855 0.27 -1.7
Or see this region's nucleotide sequence