Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09000 and PS417_09005 are separated by 10 nucleotides PS417_09005 and PS417_09010 overlap by 4 nucleotides PS417_09010 and PS417_09015 are separated by 85 nucleotides PS417_09015 and PS417_09020 are separated by 30 nucleotides PS417_09020 and PS417_09025 are separated by 97 nucleotides
PS417_09000: PS417_09000 - glycerol-3-phosphate dehydrogenase, at 1,961,083 to 1,962,108
_09000
PS417_09005: PS417_09005 - lipase, at 1,962,119 to 1,962,466
_09005
PS417_09010: PS417_09010 - phosphohistidine phosphatase, at 1,962,463 to 1,962,912
_09010
PS417_09015: PS417_09015 - AMP-binding protein, at 1,962,998 to 1,964,647
_09015
PS417_09020: PS417_09020 - esterase, at 1,964,678 to 1,965,121
_09020
PS417_09025: PS417_09025 - alpha/beta hydrolase, at 1,965,219 to 1,966,022
_09025
Position (kb)
1962
1963
1964
1965 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1962.146 kb on + strand at 1962.147 kb on - strand at 1962.249 kb on - strand, within PS417_09005 at 1962.614 kb on - strand, within PS417_09010 at 1962.656 kb on + strand, within PS417_09010 at 1962.656 kb on + strand, within PS417_09010 at 1962.657 kb on - strand, within PS417_09010 at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.938 kb on - strand at 1962.938 kb on - strand at 1962.938 kb on - strand at 1962.938 kb on - strand at 1962.939 kb on + strand at 1962.939 kb on + strand at 1962.940 kb on - strand at 1962.940 kb on - strand at 1962.940 kb on - strand at 1962.940 kb on - strand at 1963.108 kb on + strand at 1963.382 kb on - strand, within PS417_09015 at 1963.382 kb on - strand, within PS417_09015 at 1963.514 kb on + strand, within PS417_09015 at 1963.514 kb on + strand, within PS417_09015 at 1963.515 kb on - strand, within PS417_09015 at 1963.515 kb on - strand, within PS417_09015 at 1963.515 kb on - strand, within PS417_09015 at 1963.516 kb on + strand, within PS417_09015 at 1963.517 kb on - strand, within PS417_09015 at 1963.517 kb on - strand, within PS417_09015 at 1963.634 kb on + strand, within PS417_09015 at 1964.000 kb on + strand, within PS417_09015 at 1964.000 kb on + strand, within PS417_09015 at 1964.001 kb on - strand, within PS417_09015 at 1964.154 kb on - strand, within PS417_09015 at 1964.294 kb on + strand, within PS417_09015 at 1964.458 kb on - strand, within PS417_09015 at 1964.588 kb on + strand at 1964.588 kb on + strand at 1964.828 kb on + strand, within PS417_09020 at 1964.891 kb on + strand, within PS417_09020 at 1964.899 kb on + strand, within PS417_09020 at 1964.918 kb on + strand, within PS417_09020 at 1965.153 kb on + strand at 1965.153 kb on + strand at 1965.154 kb on - strand at 1965.154 kb on - strand at 1965.154 kb on - strand at 1965.154 kb on - strand at 1965.271 kb on - strand at 1965.305 kb on + strand, within PS417_09025 at 1965.305 kb on + strand, within PS417_09025 at 1965.306 kb on - strand, within PS417_09025 at 1965.360 kb on - strand, within PS417_09025 at 1965.486 kb on + strand, within PS417_09025 at 1965.646 kb on - strand, within PS417_09025 at 1965.646 kb on - strand, within PS417_09025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,962,146 + -0.3 1,962,147 - -0.0 1,962,249 - PS417_09005 0.37 +0.7 1,962,614 - PS417_09010 0.34 -1.3 1,962,656 + PS417_09010 0.43 +0.0 1,962,656 + PS417_09010 0.43 -0.8 1,962,657 - PS417_09010 0.43 -0.7 1,962,937 + -0.2 1,962,937 + -1.5 1,962,937 + +1.7 1,962,937 + -0.4 1,962,937 + +0.1 1,962,938 - +0.0 1,962,938 - -0.7 1,962,938 - -0.7 1,962,938 - +0.1 1,962,939 + -0.5 1,962,939 + +1.7 1,962,940 - -0.4 1,962,940 - -0.3 1,962,940 - -1.1 1,962,940 - -0.8 1,963,108 + -0.1 1,963,382 - PS417_09015 0.23 +0.1 1,963,382 - PS417_09015 0.23 -0.1 1,963,514 + PS417_09015 0.31 +0.1 1,963,514 + PS417_09015 0.31 +0.9 1,963,515 - PS417_09015 0.31 -0.7 1,963,515 - PS417_09015 0.31 -0.4 1,963,515 - PS417_09015 0.31 +0.8 1,963,516 + PS417_09015 0.31 +1.8 1,963,517 - PS417_09015 0.31 +0.8 1,963,517 - PS417_09015 0.31 +1.6 1,963,634 + PS417_09015 0.39 -0.9 1,964,000 + PS417_09015 0.61 +0.7 1,964,000 + PS417_09015 0.61 +0.9 1,964,001 - PS417_09015 0.61 +1.3 1,964,154 - PS417_09015 0.70 +0.4 1,964,294 + PS417_09015 0.79 +0.4 1,964,458 - PS417_09015 0.88 -0.7 1,964,588 + -0.5 1,964,588 + +0.0 1,964,828 + PS417_09020 0.34 +0.6 1,964,891 + PS417_09020 0.48 +1.1 1,964,899 + PS417_09020 0.50 +2.4 1,964,918 + PS417_09020 0.54 +0.1 1,965,153 + -0.6 1,965,153 + -0.2 1,965,154 - -0.4 1,965,154 - -1.8 1,965,154 - +0.1 1,965,154 - +0.4 1,965,271 - +0.2 1,965,305 + PS417_09025 0.11 -0.1 1,965,305 + PS417_09025 0.11 +0.1 1,965,306 - PS417_09025 0.11 +0.5 1,965,360 - PS417_09025 0.18 +0.3 1,965,486 + PS417_09025 0.33 -0.0 1,965,646 - PS417_09025 0.53 +0.2 1,965,646 - PS417_09025 0.53 +0.1
Or see this region's nucleotide sequence