Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03470 and PS417_03475 overlap by 14 nucleotides PS417_03475 and PS417_03480 overlap by 4 nucleotides PS417_03480 and PS417_03485 overlap by 4 nucleotides
PS417_03470: PS417_03470 - ATP synthase, at 798,058 to 799,416
_03470
PS417_03475: PS417_03475 - type III secretion protein, at 799,403 to 799,831
_03475
PS417_03480: PS417_03480 - type III secretion protein, at 799,828 to 800,286
_03480
PS417_03485: PS417_03485 - type III secretion protein, at 800,283 to 801,296
_03485
Position (kb) 799
800 Strain fitness (log2 ratio) -2
-1
0
1 at 798.743 kb on + strand, within PS417_03470 at 799.015 kb on + strand, within PS417_03470 at 799.015 kb on + strand, within PS417_03470 at 799.016 kb on - strand, within PS417_03470 at 799.232 kb on - strand, within PS417_03470 at 799.402 kb on + strand at 799.402 kb on + strand at 799.403 kb on - strand at 799.617 kb on + strand, within PS417_03475 at 799.618 kb on - strand, within PS417_03475 at 799.618 kb on - strand, within PS417_03475 at 799.800 kb on + strand at 799.800 kb on + strand at 800.096 kb on - strand, within PS417_03480 at 800.610 kb on + strand, within PS417_03485 at 800.722 kb on + strand, within PS417_03485 at 800.723 kb on - strand, within PS417_03485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 798,743 + PS417_03470 0.50 +0.3 799,015 + PS417_03470 0.70 -0.8 799,015 + PS417_03470 0.70 +0.3 799,016 - PS417_03470 0.70 -0.7 799,232 - PS417_03470 0.86 +0.5 799,402 + -0.4 799,402 + -0.2 799,403 - -0.2 799,617 + PS417_03475 0.50 -1.7 799,618 - PS417_03475 0.50 -0.0 799,618 - PS417_03475 0.50 +0.7 799,800 + -1.3 799,800 + -0.7 800,096 - PS417_03480 0.58 -0.8 800,610 + PS417_03485 0.32 +0.0 800,722 + PS417_03485 0.43 +0.2 800,723 - PS417_03485 0.43 -2.6
Or see this region's nucleotide sequence