Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26165 and PS417_26170 are separated by 196 nucleotides PS417_26170 and PS417_26175 are separated by 10 nucleotides PS417_26175 and PS417_26180 are separated by 123 nucleotides
PS417_26165: PS417_26165 - aldehyde dismutase, at 5,653,035 to 5,654,234
_26165
PS417_26170: PS417_26170 - formyltetrahydrofolate deformylase, at 5,654,431 to 5,655,288
_26170
PS417_26175: PS417_26175 - sarcosine oxidase subunit gamma, at 5,655,299 to 5,655,931
_26175
PS417_26180: PS417_26180 - sarcosine oxidase subunit alpha, at 5,656,055 to 5,659,072
_26180
Position (kb)
5654
5655
5656 Strain fitness (log2 ratio)
-1
0
1 at 5653.663 kb on + strand, within PS417_26165 at 5653.663 kb on + strand, within PS417_26165 at 5653.664 kb on - strand, within PS417_26165 at 5653.819 kb on + strand, within PS417_26165 at 5653.820 kb on - strand, within PS417_26165 at 5653.948 kb on - strand, within PS417_26165 at 5654.184 kb on + strand at 5654.210 kb on - strand at 5654.296 kb on - strand at 5654.330 kb on + strand at 5654.331 kb on - strand at 5654.331 kb on - strand at 5654.333 kb on + strand at 5654.334 kb on - strand at 5654.334 kb on - strand at 5654.433 kb on - strand at 5654.618 kb on + strand, within PS417_26170 at 5654.618 kb on + strand, within PS417_26170 at 5654.619 kb on - strand, within PS417_26170 at 5654.619 kb on - strand, within PS417_26170 at 5654.658 kb on - strand, within PS417_26170 at 5654.658 kb on - strand, within PS417_26170 at 5654.658 kb on - strand, within PS417_26170 at 5654.769 kb on - strand, within PS417_26170 at 5654.868 kb on - strand, within PS417_26170 at 5654.868 kb on - strand, within PS417_26170 at 5654.967 kb on - strand, within PS417_26170 at 5655.027 kb on - strand, within PS417_26170 at 5655.027 kb on - strand, within PS417_26170 at 5655.184 kb on - strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.293 kb on + strand at 5655.486 kb on + strand, within PS417_26175 at 5655.486 kb on + strand, within PS417_26175 at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.932 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,653,663 + PS417_26165 0.52 -0.3 5,653,663 + PS417_26165 0.52 -0.2 5,653,664 - PS417_26165 0.52 -0.3 5,653,819 + PS417_26165 0.65 -1.3 5,653,820 - PS417_26165 0.65 -0.1 5,653,948 - PS417_26165 0.76 -0.3 5,654,184 + -0.4 5,654,210 - -0.2 5,654,296 - -0.2 5,654,330 + +0.6 5,654,331 - -1.1 5,654,331 - -0.3 5,654,333 + +0.1 5,654,334 - +0.2 5,654,334 - +0.4 5,654,433 - -0.2 5,654,618 + PS417_26170 0.22 +0.8 5,654,618 + PS417_26170 0.22 -0.3 5,654,619 - PS417_26170 0.22 +0.1 5,654,619 - PS417_26170 0.22 -0.9 5,654,658 - PS417_26170 0.26 +0.3 5,654,658 - PS417_26170 0.26 -0.3 5,654,658 - PS417_26170 0.26 -0.7 5,654,769 - PS417_26170 0.39 +0.1 5,654,868 - PS417_26170 0.51 +0.4 5,654,868 - PS417_26170 0.51 -1.3 5,654,967 - PS417_26170 0.62 +1.5 5,655,027 - PS417_26170 0.69 +0.9 5,655,027 - PS417_26170 0.69 +0.1 5,655,184 - PS417_26170 0.88 -0.7 5,655,188 + PS417_26170 0.88 -0.2 5,655,188 + PS417_26170 0.88 +0.9 5,655,189 - PS417_26170 0.88 +1.1 5,655,189 - PS417_26170 0.88 -0.3 5,655,189 - PS417_26170 0.88 -0.6 5,655,293 + +1.4 5,655,486 + PS417_26175 0.30 -0.7 5,655,486 + PS417_26175 0.30 -0.7 5,655,914 + +0.3 5,655,914 + -0.9 5,655,914 + -0.6 5,655,914 + -1.3 5,655,914 + +0.0 5,655,915 - -1.2 5,655,915 - +0.1 5,655,915 - +0.0 5,655,932 - -0.3
Or see this region's nucleotide sequence