Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24410 and PS417_24415 are separated by 133 nucleotides PS417_24415 and PS417_24420 are separated by 63 nucleotides PS417_24420 and PS417_24430 are separated by 206 nucleotides
PS417_24410: PS417_24410 - ligand-gated channel, at 5,299,329 to 5,301,986
_24410
PS417_24415: PS417_24415 - siderophore-interacting protein, at 5,302,120 to 5,303,118
_24415
PS417_24420: PS417_24420 - RNA polymerase sigma70, at 5,303,182 to 5,303,703
_24420
PS417_24430: PS417_24430 - 3-dehydroquinate dehydratase, at 5,303,910 to 5,304,359
_24430
Position (kb) 5302
5303
5304 Strain fitness (log2 ratio) -2
-1
0
1 at 5301.291 kb on + strand, within PS417_24410 at 5301.291 kb on + strand, within PS417_24410 at 5301.291 kb on + strand, within PS417_24410 at 5301.291 kb on + strand, within PS417_24410 at 5301.292 kb on - strand, within PS417_24410 at 5301.325 kb on + strand, within PS417_24410 at 5301.325 kb on + strand, within PS417_24410 at 5301.325 kb on + strand, within PS417_24410 at 5301.325 kb on + strand, within PS417_24410 at 5301.325 kb on + strand, within PS417_24410 at 5301.326 kb on - strand, within PS417_24410 at 5301.326 kb on - strand, within PS417_24410 at 5301.426 kb on - strand, within PS417_24410 at 5301.565 kb on + strand, within PS417_24410 at 5301.565 kb on + strand, within PS417_24410 at 5301.566 kb on - strand, within PS417_24410 at 5301.727 kb on + strand at 5301.727 kb on + strand at 5301.728 kb on - strand at 5301.728 kb on - strand at 5301.746 kb on + strand at 5302.124 kb on + strand at 5302.124 kb on + strand at 5302.125 kb on - strand at 5302.125 kb on - strand at 5302.125 kb on - strand at 5302.361 kb on - strand, within PS417_24415 at 5302.403 kb on + strand, within PS417_24415 at 5302.403 kb on + strand, within PS417_24415 at 5302.403 kb on + strand, within PS417_24415 at 5302.670 kb on + strand, within PS417_24415 at 5302.671 kb on - strand, within PS417_24415 at 5302.671 kb on - strand, within PS417_24415 at 5302.760 kb on + strand, within PS417_24415 at 5302.761 kb on - strand, within PS417_24415 at 5302.761 kb on - strand, within PS417_24415 at 5302.774 kb on - strand at 5302.932 kb on - strand, within PS417_24415 at 5303.086 kb on - strand at 5303.141 kb on + strand at 5303.142 kb on - strand at 5303.145 kb on + strand at 5303.145 kb on + strand at 5303.145 kb on + strand at 5303.146 kb on - strand at 5303.231 kb on + strand at 5303.232 kb on - strand at 5303.529 kb on - strand, within PS417_24420 at 5303.743 kb on + strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.980 kb on - strand, within PS417_24430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,301,291 + PS417_24410 0.74 +0.2 5,301,291 + PS417_24410 0.74 -0.6 5,301,291 + PS417_24410 0.74 +0.4 5,301,291 + PS417_24410 0.74 -0.4 5,301,292 - PS417_24410 0.74 -0.4 5,301,325 + PS417_24410 0.75 +0.2 5,301,325 + PS417_24410 0.75 -0.4 5,301,325 + PS417_24410 0.75 +0.2 5,301,325 + PS417_24410 0.75 -0.6 5,301,325 + PS417_24410 0.75 +0.3 5,301,326 - PS417_24410 0.75 +0.4 5,301,326 - PS417_24410 0.75 -0.2 5,301,426 - PS417_24410 0.79 +1.5 5,301,565 + PS417_24410 0.84 +0.1 5,301,565 + PS417_24410 0.84 -0.3 5,301,566 - PS417_24410 0.84 +0.6 5,301,727 + +1.2 5,301,727 + +0.7 5,301,728 - -0.6 5,301,728 - -0.7 5,301,746 + +0.6 5,302,124 + +0.2 5,302,124 + -0.5 5,302,125 - -0.2 5,302,125 - +0.6 5,302,125 - -0.1 5,302,361 - PS417_24415 0.24 -0.7 5,302,403 + PS417_24415 0.28 +0.7 5,302,403 + PS417_24415 0.28 +0.8 5,302,403 + PS417_24415 0.28 -2.1 5,302,670 + PS417_24415 0.55 -0.3 5,302,671 - PS417_24415 0.55 +1.1 5,302,671 - PS417_24415 0.55 +0.4 5,302,760 + PS417_24415 0.64 -0.5 5,302,761 - PS417_24415 0.64 -0.7 5,302,761 - PS417_24415 0.64 -0.2 5,302,774 - -1.2 5,302,932 - PS417_24415 0.81 +1.1 5,303,086 - +0.2 5,303,141 + -1.1 5,303,142 - +0.7 5,303,145 + +0.3 5,303,145 + -0.2 5,303,145 + -0.5 5,303,146 - -0.5 5,303,231 + +0.0 5,303,232 - -0.3 5,303,529 - PS417_24420 0.66 -0.5 5,303,743 + +0.3 5,303,892 - -0.1 5,303,892 - -2.1 5,303,892 - +0.5 5,303,892 - -0.8 5,303,892 - +1.2 5,303,980 - PS417_24430 0.16 +0.4
Or see this region's nucleotide sequence