Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02065 and PS417_02070 overlap by 11 nucleotides PS417_02070 and PS417_02075 are separated by 29 nucleotides PS417_02075 and PS417_02080 overlap by 8 nucleotides
PS417_02065: PS417_02065 - membrane protein, at 462,463 to 464,775
_02065
PS417_02070: PS417_02070 - membrane protein, at 464,765 to 465,427
_02070
PS417_02075: PS417_02075 - thioesterase, at 465,457 to 465,882
_02075
PS417_02080: PS417_02080 - histidine ammonia-lyase, at 465,875 to 467,416
_02080
Position (kb) 464
465
466 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 463.811 kb on + strand, within PS417_02065 at 463.812 kb on - strand, within PS417_02065 at 463.835 kb on - strand, within PS417_02065 at 463.879 kb on - strand, within PS417_02065 at 463.982 kb on - strand, within PS417_02065 at 463.982 kb on - strand, within PS417_02065 at 464.083 kb on + strand, within PS417_02065 at 464.084 kb on - strand, within PS417_02065 at 464.573 kb on - strand at 464.578 kb on + strand at 464.610 kb on + strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.828 kb on - strand at 464.828 kb on - strand at 465.330 kb on - strand, within PS417_02070 at 465.476 kb on - strand at 465.596 kb on + strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 466.142 kb on + strand, within PS417_02080 at 466.142 kb on + strand, within PS417_02080 at 466.317 kb on + strand, within PS417_02080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 463,811 + PS417_02065 0.58 -0.1 463,812 - PS417_02065 0.58 -2.8 463,835 - PS417_02065 0.59 -0.8 463,879 - PS417_02065 0.61 -1.0 463,982 - PS417_02065 0.66 -1.1 463,982 - PS417_02065 0.66 +0.1 464,083 + PS417_02065 0.70 +0.0 464,084 - PS417_02065 0.70 -0.6 464,573 - -0.4 464,578 + -1.1 464,610 + +0.0 464,611 - +2.1 464,611 - +0.1 464,611 - +1.3 464,611 - -0.8 464,828 - +0.2 464,828 - -0.4 465,330 - PS417_02070 0.85 -1.3 465,476 - -0.2 465,596 + PS417_02075 0.33 -2.2 465,597 - PS417_02075 0.33 +0.6 465,597 - PS417_02075 0.33 -0.3 465,734 + PS417_02075 0.65 +1.7 465,734 + PS417_02075 0.65 -1.0 465,734 + PS417_02075 0.65 +0.2 465,735 - PS417_02075 0.65 +1.6 465,735 - PS417_02075 0.65 -0.6 466,142 + PS417_02080 0.17 -1.2 466,142 + PS417_02080 0.17 +0.2 466,317 + PS417_02080 0.29 -1.5
Or see this region's nucleotide sequence