Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11665 and PS417_11670 are separated by 55 nucleotides PS417_11670 and PS417_11675 overlap by 17 nucleotides PS417_11675 and PS417_11680 are separated by 140 nucleotides
PS417_11665: PS417_11665 - ATPase AAA, at 2,509,964 to 2,510,929
_11665
PS417_11670: PS417_11670 - multidrug transporter MATE, at 2,510,985 to 2,512,340
_11670
PS417_11675: PS417_11675 - TetR family transcriptional regulator, at 2,512,324 to 2,512,911
_11675
PS417_11680: PS417_11680 - MFS transporter, at 2,513,052 to 2,514,224
_11680
Position (kb)
2510
2511
2512
2513 Strain fitness (log2 ratio)
-1
0
1 at 2510.519 kb on - strand, within PS417_11665 at 2510.865 kb on + strand at 2510.938 kb on + strand at 2510.944 kb on - strand at 2510.962 kb on - strand at 2511.093 kb on + strand at 2511.094 kb on - strand at 2511.843 kb on + strand, within PS417_11670 at 2511.844 kb on - strand, within PS417_11670 at 2511.996 kb on + strand, within PS417_11670 at 2512.206 kb on + strand at 2512.206 kb on + strand at 2512.206 kb on + strand at 2512.914 kb on + strand at 2512.980 kb on - strand at 2513.105 kb on - strand at 2513.216 kb on - strand, within PS417_11680 at 2513.216 kb on - strand, within PS417_11680 at 2513.216 kb on - strand, within PS417_11680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,510,519 - PS417_11665 0.57 -0.2 2,510,865 + +0.3 2,510,938 + +0.3 2,510,944 - +0.9 2,510,962 - +0.8 2,511,093 + +1.2 2,511,094 - +1.2 2,511,843 + PS417_11670 0.63 +1.2 2,511,844 - PS417_11670 0.63 +1.2 2,511,996 + PS417_11670 0.75 +1.0 2,512,206 + +0.9 2,512,206 + +1.3 2,512,206 + +0.9 2,512,914 + +0.2 2,512,980 - -0.2 2,513,105 - +0.0 2,513,216 - PS417_11680 0.14 +0.1 2,513,216 - PS417_11680 0.14 -0.6 2,513,216 - PS417_11680 0.14 +0.2
Or see this region's nucleotide sequence