Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04790 and PS417_04795 are separated by 173 nucleotides PS417_04795 and PS417_04800 are separated by 16 nucleotides PS417_04800 and PS417_04805 are separated by 11 nucleotides
PS417_04790: PS417_04790 - mannose-1-phosphate guanylyltransferase, at 1,060,574 to 1,062,025
_04790
PS417_04795: PS417_04795 - alginate O-acetyltransferase, at 1,062,199 to 1,062,855
_04795
PS417_04800: PS417_04800 - alginate O-acetyltransferase, at 1,062,872 to 1,064,056
_04800
PS417_04805: PS417_04805 - poly(beta-D-mannuronate) O-acetylase, at 1,064,068 to 1,065,600
_04805
Position (kb)
1062
1063
1064
1065 Strain fitness (log2 ratio)
-2
-1
0
1 at 1062.196 kb on + strand at 1062.196 kb on + strand at 1062.197 kb on - strand at 1062.263 kb on + strand at 1062.264 kb on - strand at 1062.332 kb on + strand, within PS417_04795 at 1062.333 kb on - strand, within PS417_04795 at 1062.333 kb on - strand, within PS417_04795 at 1062.545 kb on + strand, within PS417_04795 at 1062.545 kb on + strand, within PS417_04795 at 1062.545 kb on + strand, within PS417_04795 at 1062.545 kb on + strand, within PS417_04795 at 1062.546 kb on - strand, within PS417_04795 at 1062.546 kb on - strand, within PS417_04795 at 1062.602 kb on + strand, within PS417_04795 at 1062.707 kb on + strand, within PS417_04795 at 1062.708 kb on - strand, within PS417_04795 at 1062.708 kb on - strand, within PS417_04795 at 1062.843 kb on + strand at 1062.862 kb on + strand at 1063.018 kb on - strand at 1063.194 kb on + strand, within PS417_04800 at 1063.387 kb on + strand, within PS417_04800 at 1063.388 kb on - strand, within PS417_04800 at 1063.846 kb on - strand, within PS417_04800 at 1064.031 kb on + strand at 1064.031 kb on + strand at 1064.031 kb on + strand at 1064.032 kb on - strand at 1064.082 kb on - strand at 1064.186 kb on + strand at 1064.448 kb on - strand, within PS417_04805 at 1064.448 kb on - strand, within PS417_04805 at 1064.448 kb on - strand, within PS417_04805 at 1064.451 kb on + strand, within PS417_04805 at 1064.618 kb on + strand, within PS417_04805 at 1064.619 kb on - strand, within PS417_04805 at 1064.619 kb on - strand, within PS417_04805 at 1064.720 kb on + strand, within PS417_04805 at 1064.721 kb on - strand, within PS417_04805 at 1064.721 kb on - strand, within PS417_04805 at 1064.721 kb on - strand, within PS417_04805 at 1064.721 kb on - strand, within PS417_04805 at 1064.721 kb on - strand, within PS417_04805 at 1064.721 kb on - strand, within PS417_04805 at 1064.726 kb on + strand, within PS417_04805 at 1064.727 kb on - strand, within PS417_04805 at 1064.795 kb on + strand, within PS417_04805 at 1064.795 kb on + strand, within PS417_04805 at 1064.796 kb on - strand, within PS417_04805 at 1064.883 kb on - strand at 1064.883 kb on - strand, within PS417_04805 at 1064.897 kb on + strand, within PS417_04805 at 1064.897 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,062,196 + -0.9 1,062,196 + -1.6 1,062,197 - +1.0 1,062,263 + -0.4 1,062,264 - +0.3 1,062,332 + PS417_04795 0.20 +0.0 1,062,333 - PS417_04795 0.20 +0.3 1,062,333 - PS417_04795 0.20 -0.3 1,062,545 + PS417_04795 0.53 +0.0 1,062,545 + PS417_04795 0.53 -0.1 1,062,545 + PS417_04795 0.53 -0.1 1,062,545 + PS417_04795 0.53 -0.3 1,062,546 - PS417_04795 0.53 -0.4 1,062,546 - PS417_04795 0.53 +0.4 1,062,602 + PS417_04795 0.61 -0.2 1,062,707 + PS417_04795 0.77 +0.0 1,062,708 - PS417_04795 0.77 +0.1 1,062,708 - PS417_04795 0.77 +0.6 1,062,843 + +0.2 1,062,862 + +0.2 1,063,018 - -0.1 1,063,194 + PS417_04800 0.27 +0.6 1,063,387 + PS417_04800 0.43 +0.7 1,063,388 - PS417_04800 0.44 -0.5 1,063,846 - PS417_04800 0.82 -0.3 1,064,031 + -0.2 1,064,031 + -0.5 1,064,031 + -0.2 1,064,032 - +0.1 1,064,082 - -0.5 1,064,186 + +0.4 1,064,448 - PS417_04805 0.25 -0.4 1,064,448 - PS417_04805 0.25 +0.3 1,064,448 - PS417_04805 0.25 +0.7 1,064,451 + PS417_04805 0.25 -0.3 1,064,618 + PS417_04805 0.36 -0.7 1,064,619 - PS417_04805 0.36 -1.2 1,064,619 - PS417_04805 0.36 +0.9 1,064,720 + PS417_04805 0.43 -0.5 1,064,721 - PS417_04805 0.43 -1.6 1,064,721 - PS417_04805 0.43 +0.2 1,064,721 - PS417_04805 0.43 -0.3 1,064,721 - PS417_04805 0.43 -0.1 1,064,721 - PS417_04805 0.43 -0.1 1,064,721 - PS417_04805 0.43 -0.5 1,064,726 + PS417_04805 0.43 +0.7 1,064,727 - PS417_04805 0.43 -0.1 1,064,795 + PS417_04805 0.47 -0.1 1,064,795 + PS417_04805 0.47 -0.1 1,064,796 - PS417_04805 0.47 -0.3 1,064,883 - +0.4 1,064,883 - PS417_04805 0.53 +0.3 1,064,897 + PS417_04805 0.54 -2.4 1,064,897 + +0.7
Or see this region's nucleotide sequence