Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28405 and PS417_28410 are separated by 9 nucleotides PS417_28410 and PS417_28415 are separated by 1 nucleotides PS417_28415 and PS417_28420 are separated by 22 nucleotides PS417_28420 and PS417_28425 are separated by 150 nucleotides
PS417_28405: PS417_28405 - hypothetical protein, at 6,132,434 to 6,132,877
_28405
PS417_28410: PS417_28410 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 6,132,887 to 6,133,507
_28410
PS417_28415: PS417_28415 - phosphatidate cytidylyltransferase, at 6,133,509 to 6,134,441
_28415
PS417_28420: PS417_28420 - AsnC family transcriptional regulator, at 6,134,464 to 6,134,937
_28420
PS417_28425: PS417_28425 - lysine transporter LysE, at 6,135,088 to 6,135,717
_28425
Position (kb)
6133
6134
6135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6132.531 kb on + strand, within PS417_28405 at 6132.531 kb on + strand, within PS417_28405 at 6132.531 kb on + strand, within PS417_28405 at 6132.532 kb on - strand, within PS417_28405 at 6132.624 kb on - strand, within PS417_28405 at 6132.886 kb on + strand at 6132.887 kb on - strand at 6132.992 kb on + strand, within PS417_28410 at 6132.993 kb on - strand, within PS417_28410 at 6132.993 kb on - strand, within PS417_28410 at 6133.091 kb on + strand, within PS417_28410 at 6133.118 kb on + strand, within PS417_28410 at 6133.118 kb on + strand, within PS417_28410 at 6133.119 kb on - strand, within PS417_28410 at 6133.351 kb on - strand, within PS417_28410 at 6133.378 kb on - strand, within PS417_28410 at 6133.591 kb on + strand at 6133.591 kb on + strand at 6133.592 kb on - strand at 6133.592 kb on - strand at 6133.666 kb on + strand, within PS417_28415 at 6133.734 kb on + strand, within PS417_28415 at 6133.734 kb on + strand, within PS417_28415 at 6133.734 kb on + strand, within PS417_28415 at 6133.735 kb on - strand, within PS417_28415 at 6133.827 kb on + strand, within PS417_28415 at 6133.827 kb on + strand, within PS417_28415 at 6133.828 kb on - strand, within PS417_28415 at 6133.828 kb on - strand, within PS417_28415 at 6133.839 kb on + strand, within PS417_28415 at 6133.881 kb on + strand, within PS417_28415 at 6133.882 kb on - strand, within PS417_28415 at 6133.882 kb on - strand, within PS417_28415 at 6134.062 kb on - strand, within PS417_28415 at 6134.092 kb on + strand, within PS417_28415 at 6134.092 kb on + strand, within PS417_28415 at 6134.092 kb on + strand, within PS417_28415 at 6134.093 kb on - strand, within PS417_28415 at 6134.100 kb on + strand, within PS417_28415 at 6134.100 kb on + strand, within PS417_28415 at 6134.101 kb on - strand, within PS417_28415 at 6134.101 kb on - strand, within PS417_28415 at 6134.226 kb on - strand, within PS417_28415 at 6134.345 kb on + strand, within PS417_28415 at 6134.346 kb on - strand, within PS417_28415 at 6134.448 kb on + strand at 6135.085 kb on + strand at 6135.085 kb on + strand at 6135.130 kb on + strand at 6135.342 kb on + strand, within PS417_28425 at 6135.363 kb on + strand, within PS417_28425 at 6135.363 kb on + strand, within PS417_28425 at 6135.364 kb on - strand, within PS417_28425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 6,132,531 + PS417_28405 0.22 -1.0 6,132,531 + PS417_28405 0.22 -1.2 6,132,531 + PS417_28405 0.22 +0.5 6,132,532 - PS417_28405 0.22 +1.3 6,132,624 - PS417_28405 0.43 +0.1 6,132,886 + -0.3 6,132,887 - +0.2 6,132,992 + PS417_28410 0.17 +0.3 6,132,993 - PS417_28410 0.17 +0.4 6,132,993 - PS417_28410 0.17 -0.6 6,133,091 + PS417_28410 0.33 +0.5 6,133,118 + PS417_28410 0.37 +0.3 6,133,118 + PS417_28410 0.37 +0.3 6,133,119 - PS417_28410 0.37 +1.6 6,133,351 - PS417_28410 0.75 -1.2 6,133,378 - PS417_28410 0.79 +0.2 6,133,591 + +0.5 6,133,591 + +0.1 6,133,592 - -1.1 6,133,592 - -1.3 6,133,666 + PS417_28415 0.17 +0.5 6,133,734 + PS417_28415 0.24 +1.8 6,133,734 + PS417_28415 0.24 +0.2 6,133,734 + PS417_28415 0.24 -0.1 6,133,735 - PS417_28415 0.24 -2.1 6,133,827 + PS417_28415 0.34 +0.7 6,133,827 + PS417_28415 0.34 +0.3 6,133,828 - PS417_28415 0.34 +0.6 6,133,828 - PS417_28415 0.34 -0.2 6,133,839 + PS417_28415 0.35 -0.0 6,133,881 + PS417_28415 0.40 +1.3 6,133,882 - PS417_28415 0.40 -0.4 6,133,882 - PS417_28415 0.40 +0.7 6,134,062 - PS417_28415 0.59 +0.2 6,134,092 + PS417_28415 0.62 +0.1 6,134,092 + PS417_28415 0.62 +0.7 6,134,092 + PS417_28415 0.62 +0.5 6,134,093 - PS417_28415 0.63 +0.6 6,134,100 + PS417_28415 0.63 -0.3 6,134,100 + PS417_28415 0.63 +1.2 6,134,101 - PS417_28415 0.63 +0.6 6,134,101 - PS417_28415 0.63 +0.2 6,134,226 - PS417_28415 0.77 +0.3 6,134,345 + PS417_28415 0.90 +0.3 6,134,346 - PS417_28415 0.90 +0.5 6,134,448 + -0.8 6,135,085 + +0.4 6,135,085 + +0.8 6,135,130 + -0.1 6,135,342 + PS417_28425 0.40 +0.6 6,135,363 + PS417_28425 0.44 -0.2 6,135,363 + PS417_28425 0.44 -1.0 6,135,364 - PS417_28425 0.44 +0.0
Or see this region's nucleotide sequence