Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02740 and PS417_02745 are separated by 136 nucleotides PS417_02745 and PS417_02750 are separated by 80 nucleotides PS417_02750 and PS417_02755 overlap by 4 nucleotides
PS417_02740: PS417_02740 - LysR family transcriptional regulator, at 624,631 to 625,530
_02740
PS417_02745: PS417_02745 - FMN-dependent NADH-azoreductase, at 625,667 to 626,308
_02745
PS417_02750: PS417_02750 - alpha/beta hydrolase, at 626,389 to 627,279
_02750
PS417_02755: PS417_02755 - membrane protein, at 627,276 to 628,145
_02755
Position (kb)
625
626
627 Strain fitness (log2 ratio)
-1
0
1 at 625.028 kb on + strand, within PS417_02740 at 625.028 kb on + strand, within PS417_02740 at 625.029 kb on - strand, within PS417_02740 at 625.663 kb on - strand at 626.186 kb on + strand, within PS417_02745 at 626.187 kb on - strand, within PS417_02745 at 626.187 kb on - strand at 626.247 kb on + strand at 626.247 kb on + strand at 626.248 kb on - strand at 626.248 kb on - strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.262 kb on - strand at 626.307 kb on - strand at 626.347 kb on + strand at 626.348 kb on - strand at 626.358 kb on + strand at 626.358 kb on + strand at 626.359 kb on - strand at 626.359 kb on - strand at 626.359 kb on - strand at 626.361 kb on + strand at 626.361 kb on + strand at 626.361 kb on + strand at 626.362 kb on - strand at 626.556 kb on - strand, within PS417_02750 at 626.556 kb on - strand, within PS417_02750 at 626.756 kb on - strand, within PS417_02750 at 626.923 kb on + strand, within PS417_02750 at 626.990 kb on - strand, within PS417_02750 at 627.127 kb on + strand, within PS417_02750 at 627.128 kb on - strand, within PS417_02750 at 627.163 kb on + strand, within PS417_02750 at 627.164 kb on - strand, within PS417_02750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 625,028 + PS417_02740 0.44 -0.4 625,028 + PS417_02740 0.44 -0.5 625,029 - PS417_02740 0.44 -1.1 625,663 - -0.2 626,186 + PS417_02745 0.81 +0.6 626,187 - PS417_02745 0.81 +0.1 626,187 - -0.8 626,247 + -0.3 626,247 + +0.5 626,248 - -0.1 626,248 - -0.5 626,261 + +0.2 626,261 + +0.5 626,262 - +0.6 626,307 - +0.0 626,347 + -0.1 626,348 - +0.3 626,358 + +0.2 626,358 + -1.3 626,359 - -0.8 626,359 - +0.0 626,359 - +0.1 626,361 + +0.7 626,361 + +1.0 626,361 + -1.1 626,362 - +1.5 626,556 - PS417_02750 0.19 -0.1 626,556 - PS417_02750 0.19 -0.2 626,756 - PS417_02750 0.41 -0.0 626,923 + PS417_02750 0.60 +0.2 626,990 - PS417_02750 0.67 +0.2 627,127 + PS417_02750 0.83 -0.7 627,128 - PS417_02750 0.83 +0.8 627,163 + PS417_02750 0.87 -0.2 627,164 - PS417_02750 0.87 +0.1
Or see this region's nucleotide sequence