Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02480 and PS417_02485 overlap by 1 nucleotides PS417_02485 and PS417_02490 are separated by 95 nucleotides PS417_02490 and PS417_02495 are separated by 12 nucleotides
PS417_02480: PS417_02480 - DNA mismatch repair protein, at 566,499 to 568,415
_02480
PS417_02485: PS417_02485 - tRNA delta(2)-isopentenylpyrophosphate transferase, at 568,415 to 569,386
_02485
PS417_02490: PS417_02490 - RNA-binding protein Hfq, at 569,482 to 569,742
_02490
PS417_02495: PS417_02495 - GTPase HflX, at 569,755 to 571,056
_02495
Position (kb) 568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 567.660 kb on + strand, within PS417_02480 at 567.661 kb on - strand, within PS417_02480 at 567.700 kb on - strand, within PS417_02480 at 567.705 kb on + strand, within PS417_02480 at 567.706 kb on - strand, within PS417_02480 at 567.830 kb on + strand, within PS417_02480 at 567.873 kb on + strand, within PS417_02480 at 567.874 kb on - strand, within PS417_02480 at 567.874 kb on - strand, within PS417_02480 at 568.171 kb on + strand, within PS417_02480 at 568.700 kb on - strand, within PS417_02485 at 568.731 kb on - strand, within PS417_02485 at 569.016 kb on - strand, within PS417_02485 at 569.018 kb on - strand, within PS417_02485 at 569.092 kb on + strand, within PS417_02485 at 569.093 kb on - strand, within PS417_02485 at 569.124 kb on + strand, within PS417_02485 at 569.179 kb on - strand, within PS417_02485 at 569.466 kb on + strand at 569.733 kb on + strand at 569.743 kb on + strand at 569.744 kb on - strand at 569.744 kb on - strand at 569.744 kb on - strand at 569.898 kb on - strand, within PS417_02495 at 570.028 kb on - strand, within PS417_02495 at 570.370 kb on + strand, within PS417_02495 at 570.373 kb on - strand, within PS417_02495
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 567,660 + PS417_02480 0.61 -0.3 567,661 - PS417_02480 0.61 -0.9 567,700 - PS417_02480 0.63 -0.7 567,705 + PS417_02480 0.63 -0.5 567,706 - PS417_02480 0.63 -1.5 567,830 + PS417_02480 0.69 -0.3 567,873 + PS417_02480 0.72 +0.9 567,874 - PS417_02480 0.72 -1.7 567,874 - PS417_02480 0.72 -1.1 568,171 + PS417_02480 0.87 -0.0 568,700 - PS417_02485 0.29 -0.3 568,731 - PS417_02485 0.33 -1.1 569,016 - PS417_02485 0.62 -3.1 569,018 - PS417_02485 0.62 -0.1 569,092 + PS417_02485 0.70 -1.1 569,093 - PS417_02485 0.70 -2.1 569,124 + PS417_02485 0.73 -1.3 569,179 - PS417_02485 0.79 +0.1 569,466 + -1.5 569,733 + -1.8 569,743 + -0.8 569,744 - -0.9 569,744 - -1.2 569,744 - +1.0 569,898 - PS417_02495 0.11 -0.6 570,028 - PS417_02495 0.21 +1.5 570,370 + PS417_02495 0.47 -0.5 570,373 - PS417_02495 0.47 -0.1
Or see this region's nucleotide sequence