Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18980 and PS417_18990 are separated by 301 nucleotides PS417_18990 and PS417_18995 are separated by 57 nucleotides PS417_18995 and PS417_19000 are separated by 94 nucleotides
PS417_18980: PS417_18980 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 4,109,010 to 4,109,825
_18980
PS417_18990: PS417_18990 - membrane protein, at 4,110,127 to 4,111,338
_18990
PS417_18995: PS417_18995 - endonuclease, at 4,111,396 to 4,112,190
_18995
PS417_19000: PS417_19000 - MFS transporter, at 4,112,285 to 4,113,502
_19000
Position (kb)
4110
4111
4112 Strain fitness (log2 ratio)
-2
-1
0
1 at 4109.546 kb on - strand, within PS417_18980 at 4109.546 kb on - strand, within PS417_18980 at 4109.738 kb on - strand, within PS417_18980 at 4109.945 kb on - strand at 4110.051 kb on + strand at 4110.052 kb on - strand at 4110.052 kb on - strand at 4110.052 kb on - strand at 4110.441 kb on + strand, within PS417_18990 at 4110.441 kb on + strand, within PS417_18990 at 4110.441 kb on + strand, within PS417_18990 at 4110.442 kb on - strand, within PS417_18990 at 4110.564 kb on + strand, within PS417_18990 at 4110.602 kb on + strand, within PS417_18990 at 4111.385 kb on - strand at 4111.386 kb on + strand at 4111.386 kb on + strand at 4111.387 kb on - strand at 4111.473 kb on - strand at 4111.517 kb on + strand, within PS417_18995 at 4111.517 kb on + strand, within PS417_18995 at 4111.517 kb on + strand, within PS417_18995 at 4111.517 kb on + strand, within PS417_18995 at 4111.518 kb on - strand, within PS417_18995 at 4111.547 kb on + strand, within PS417_18995 at 4111.637 kb on + strand, within PS417_18995 at 4111.638 kb on - strand, within PS417_18995 at 4111.974 kb on - strand, within PS417_18995 at 4111.974 kb on - strand, within PS417_18995 at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.187 kb on - strand at 4112.187 kb on - strand at 4112.187 kb on - strand at 4112.195 kb on + strand at 4112.195 kb on + strand at 4112.195 kb on + strand at 4112.196 kb on - strand at 4112.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 4,109,546 - PS417_18980 0.66 -0.5 4,109,546 - PS417_18980 0.66 +0.2 4,109,738 - PS417_18980 0.89 -0.1 4,109,945 - -0.0 4,110,051 + +1.4 4,110,052 - +0.0 4,110,052 - -0.2 4,110,052 - +0.8 4,110,441 + PS417_18990 0.26 -2.7 4,110,441 + PS417_18990 0.26 -0.9 4,110,441 + PS417_18990 0.26 -1.2 4,110,442 - PS417_18990 0.26 -0.0 4,110,564 + PS417_18990 0.36 -1.2 4,110,602 + PS417_18990 0.39 -0.5 4,111,385 - +0.3 4,111,386 + +0.0 4,111,386 + -0.2 4,111,387 - -1.9 4,111,473 - +0.2 4,111,517 + PS417_18995 0.15 -1.4 4,111,517 + PS417_18995 0.15 -0.5 4,111,517 + PS417_18995 0.15 -1.4 4,111,517 + PS417_18995 0.15 -0.4 4,111,518 - PS417_18995 0.15 -0.1 4,111,547 + PS417_18995 0.19 +0.8 4,111,637 + PS417_18995 0.30 +0.2 4,111,638 - PS417_18995 0.30 +0.0 4,111,974 - PS417_18995 0.73 -0.3 4,111,974 - PS417_18995 0.73 +0.0 4,112,186 + -0.1 4,112,186 + +0.3 4,112,186 + +0.0 4,112,186 + -0.3 4,112,186 + -0.2 4,112,187 - +0.0 4,112,187 - +0.7 4,112,187 - +0.8 4,112,195 + +0.4 4,112,195 + -0.4 4,112,195 + -0.0 4,112,196 - +0.4 4,112,265 - +0.4
Or see this region's nucleotide sequence