Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01790 and PS417_01795 overlap by 8 nucleotides PS417_01795 and PS417_01800 are separated by 186 nucleotides
PS417_01790: PS417_01790 - glucan biosynthesis protein G, at 397,546 to 399,282
_01790
PS417_01795: PS417_01795 - glucosyltransferase MdoH, at 399,275 to 401,845
_01795
PS417_01800: PS417_01800 - amino acid ABC transporter substrate-binding protein, at 402,032 to 402,832
_01800
Position (kb) 399
400
401
402 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 398.350 kb on + strand, within PS417_01790 at 398.351 kb on - strand, within PS417_01790 at 398.351 kb on - strand, within PS417_01790 at 398.351 kb on - strand, within PS417_01790 at 398.389 kb on + strand, within PS417_01790 at 398.576 kb on - strand, within PS417_01790 at 398.576 kb on - strand, within PS417_01790 at 398.576 kb on - strand, within PS417_01790 at 398.869 kb on + strand, within PS417_01790 at 398.870 kb on - strand, within PS417_01790 at 399.259 kb on + strand at 399.259 kb on + strand at 399.259 kb on + strand at 399.280 kb on + strand at 399.281 kb on - strand at 399.315 kb on - strand at 399.328 kb on - strand at 399.657 kb on + strand, within PS417_01795 at 399.658 kb on - strand, within PS417_01795 at 399.749 kb on + strand, within PS417_01795 at 399.750 kb on - strand, within PS417_01795 at 399.840 kb on + strand, within PS417_01795 at 399.958 kb on + strand, within PS417_01795 at 399.958 kb on + strand, within PS417_01795 at 399.959 kb on - strand, within PS417_01795 at 400.144 kb on + strand, within PS417_01795 at 400.144 kb on + strand, within PS417_01795 at 400.144 kb on + strand, within PS417_01795 at 400.227 kb on - strand, within PS417_01795 at 400.307 kb on + strand, within PS417_01795 at 400.307 kb on + strand, within PS417_01795 at 400.308 kb on + strand, within PS417_01795 at 400.308 kb on - strand, within PS417_01795 at 400.308 kb on - strand, within PS417_01795 at 400.308 kb on - strand, within PS417_01795 at 400.439 kb on + strand, within PS417_01795 at 400.472 kb on + strand, within PS417_01795 at 400.703 kb on + strand, within PS417_01795 at 400.847 kb on + strand, within PS417_01795 at 400.953 kb on - strand, within PS417_01795 at 401.173 kb on - strand, within PS417_01795 at 401.450 kb on + strand, within PS417_01795 at 401.486 kb on + strand, within PS417_01795 at 401.487 kb on - strand, within PS417_01795 at 401.487 kb on - strand, within PS417_01795 at 401.487 kb on - strand, within PS417_01795 at 401.487 kb on - strand, within PS417_01795 at 401.895 kb on + strand at 402.006 kb on + strand at 402.045 kb on - strand at 402.063 kb on + strand at 402.063 kb on + strand at 402.161 kb on + strand, within PS417_01800 at 402.162 kb on - strand, within PS417_01800 at 402.162 kb on - strand, within PS417_01800 at 402.162 kb on - strand, within PS417_01800 at 402.162 kb on - strand, within PS417_01800 at 402.162 kb on - strand, within PS417_01800 at 402.162 kb on - strand at 402.598 kb on + strand, within PS417_01800 at 402.598 kb on + strand, within PS417_01800 at 402.598 kb on + strand, within PS417_01800 at 402.614 kb on + strand, within PS417_01800 at 402.728 kb on - strand, within PS417_01800 at 402.773 kb on + strand at 402.774 kb on - strand at 402.774 kb on - strand at 402.774 kb on - strand at 402.774 kb on - strand at 402.839 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 398,350 + PS417_01790 0.46 -1.2 398,351 - PS417_01790 0.46 +0.4 398,351 - PS417_01790 0.46 -1.0 398,351 - PS417_01790 0.46 +1.2 398,389 + PS417_01790 0.49 -0.8 398,576 - PS417_01790 0.59 -0.9 398,576 - PS417_01790 0.59 -1.4 398,576 - PS417_01790 0.59 -1.5 398,869 + PS417_01790 0.76 -0.8 398,870 - PS417_01790 0.76 -0.7 399,259 + -0.3 399,259 + -0.3 399,259 + -0.1 399,280 + -0.7 399,281 - -1.1 399,315 - +3.1 399,328 - +0.5 399,657 + PS417_01795 0.15 -0.5 399,658 - PS417_01795 0.15 -2.0 399,749 + PS417_01795 0.18 -0.9 399,750 - PS417_01795 0.18 +1.2 399,840 + PS417_01795 0.22 -0.9 399,958 + PS417_01795 0.27 -1.5 399,958 + PS417_01795 0.27 -0.2 399,959 - PS417_01795 0.27 -0.9 400,144 + PS417_01795 0.34 -1.2 400,144 + PS417_01795 0.34 -0.4 400,144 + PS417_01795 0.34 -0.7 400,227 - PS417_01795 0.37 +0.7 400,307 + PS417_01795 0.40 -1.3 400,307 + PS417_01795 0.40 +0.5 400,308 + PS417_01795 0.40 -0.4 400,308 - PS417_01795 0.40 +1.4 400,308 - PS417_01795 0.40 -0.2 400,308 - PS417_01795 0.40 -0.9 400,439 + PS417_01795 0.45 -0.2 400,472 + PS417_01795 0.47 -0.8 400,703 + PS417_01795 0.56 -1.2 400,847 + PS417_01795 0.61 -0.4 400,953 - PS417_01795 0.65 -0.3 401,173 - PS417_01795 0.74 -0.3 401,450 + PS417_01795 0.85 +0.0 401,486 + PS417_01795 0.86 -0.1 401,487 - PS417_01795 0.86 -1.0 401,487 - PS417_01795 0.86 -0.1 401,487 - PS417_01795 0.86 -2.2 401,487 - PS417_01795 0.86 -0.7 401,895 + +0.4 402,006 + +1.2 402,045 - +0.5 402,063 + +0.5 402,063 + +0.3 402,161 + PS417_01800 0.16 +0.1 402,162 - PS417_01800 0.16 -0.1 402,162 - PS417_01800 0.16 -1.7 402,162 - PS417_01800 0.16 +0.6 402,162 - PS417_01800 0.16 +1.2 402,162 - PS417_01800 0.16 -0.2 402,162 - +1.9 402,598 + PS417_01800 0.71 +0.9 402,598 + PS417_01800 0.71 -0.3 402,598 + PS417_01800 0.71 +1.5 402,614 + PS417_01800 0.73 -0.4 402,728 - PS417_01800 0.87 +0.8 402,773 + +0.3 402,774 - -2.1 402,774 - -0.5 402,774 - +1.0 402,774 - -0.2 402,839 + -1.8
Or see this region's nucleotide sequence