Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16790 and PS417_16795 overlap by 4 nucleotides PS417_16795 and PS417_16800 overlap by 4 nucleotides PS417_16800 and PS417_16805 are separated by 123 nucleotides
PS417_16790: PS417_16790 - ABC transporter permease, at 3,663,076 to 3,663,957
_16790
PS417_16795: PS417_16795 - ABC transporter, at 3,663,954 to 3,664,727
_16795
PS417_16800: PS417_16800 - acyl-CoA synthetase, at 3,664,724 to 3,665,626
_16800
PS417_16805: PS417_16805 - 5,10-methylene tetrahydromethanopterin reductase, at 3,665,750 to 3,667,129
_16805
Position (kb)
3664
3665
3666 Strain fitness (log2 ratio)
-1
0
1 at 3663.906 kb on - strand at 3663.906 kb on - strand at 3663.977 kb on - strand at 3664.132 kb on - strand, within PS417_16795 at 3664.794 kb on + strand at 3664.794 kb on + strand at 3664.795 kb on - strand at 3664.795 kb on - strand at 3664.795 kb on - strand at 3665.241 kb on + strand, within PS417_16800 at 3665.241 kb on + strand, within PS417_16800 at 3665.241 kb on + strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.693 kb on - strand at 3665.693 kb on - strand at 3665.778 kb on + strand at 3665.854 kb on - strand at 3665.943 kb on - strand, within PS417_16805 at 3665.943 kb on - strand, within PS417_16805 at 3666.225 kb on + strand, within PS417_16805 at 3666.398 kb on + strand, within PS417_16805 at 3666.624 kb on + strand, within PS417_16805 at 3666.625 kb on - strand, within PS417_16805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,663,906 - +0.9 3,663,906 - +0.1 3,663,977 - -0.8 3,664,132 - PS417_16795 0.23 -0.7 3,664,794 + +0.1 3,664,794 + +0.5 3,664,795 - -0.7 3,664,795 - +0.0 3,664,795 - -0.0 3,665,241 + PS417_16800 0.57 +1.2 3,665,241 + PS417_16800 0.57 +1.1 3,665,241 + PS417_16800 0.57 +0.5 3,665,242 - PS417_16800 0.57 +0.2 3,665,242 - PS417_16800 0.57 -0.3 3,665,242 - PS417_16800 0.57 -0.0 3,665,242 - PS417_16800 0.57 -0.0 3,665,242 - PS417_16800 0.57 +0.8 3,665,242 - PS417_16800 0.57 -0.1 3,665,692 + -1.1 3,665,692 + +0.1 3,665,692 + -0.1 3,665,693 - -0.0 3,665,693 - +0.3 3,665,778 + -0.2 3,665,854 - +0.3 3,665,943 - PS417_16805 0.14 -0.1 3,665,943 - PS417_16805 0.14 +0.2 3,666,225 + PS417_16805 0.34 -1.0 3,666,398 + PS417_16805 0.47 -0.2 3,666,624 + PS417_16805 0.63 +0.4 3,666,625 - PS417_16805 0.63 -0.2
Or see this region's nucleotide sequence