Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15510 and PS417_15515 are separated by 90 nucleotides PS417_15515 and PS417_15520 are separated by 23 nucleotides PS417_15520 and PS417_15525 are separated by 120 nucleotides PS417_15525 and PS417_15530 overlap by 10 nucleotides PS417_15530 and PS417_15535 are separated by 36 nucleotides
PS417_15510: PS417_15510 - hypothetical protein, at 3,397,429 to 3,397,773
_15510
PS417_15515: PS417_15515 - hypothetical protein, at 3,397,864 to 3,398,211
_15515
PS417_15520: PS417_15520 - hypothetical protein, at 3,398,235 to 3,398,645
_15520
PS417_15525: PS417_15525 - AraC family transcriptional regulator, at 3,398,766 to 3,399,632
_15525
PS417_15530: PS417_15530 - ribonuclease, at 3,399,623 to 3,400,252
_15530
PS417_15535: PS417_15535 - oxidoreductase, at 3,400,289 to 3,401,269
_15535
Position (kb)
3398
3399
3400 Strain fitness (log2 ratio)
-2
-1
0
1 at 3397.810 kb on + strand at 3398.227 kb on - strand at 3398.416 kb on + strand, within PS417_15520 at 3398.416 kb on + strand, within PS417_15520 at 3398.690 kb on + strand at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3399.039 kb on + strand, within PS417_15525 at 3399.040 kb on - strand, within PS417_15525 at 3399.040 kb on - strand, within PS417_15525 at 3399.156 kb on + strand, within PS417_15525 at 3399.156 kb on + strand, within PS417_15525 at 3399.156 kb on + strand, within PS417_15525 at 3399.157 kb on - strand, within PS417_15525 at 3399.157 kb on - strand, within PS417_15525 at 3399.157 kb on - strand, within PS417_15525 at 3399.646 kb on + strand at 3399.825 kb on + strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.928 kb on + strand, within PS417_15530 at 3399.929 kb on - strand, within PS417_15530 at 3400.053 kb on + strand, within PS417_15530 at 3400.065 kb on - strand, within PS417_15530 at 3400.076 kb on + strand, within PS417_15530 at 3400.077 kb on - strand, within PS417_15530 at 3400.077 kb on - strand, within PS417_15530 at 3400.286 kb on + strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.290 kb on + strand at 3400.316 kb on + strand at 3400.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,397,810 + +0.1 3,398,227 - -0.2 3,398,416 + PS417_15520 0.44 -0.9 3,398,416 + PS417_15520 0.44 -2.4 3,398,690 + -0.7 3,398,913 + PS417_15525 0.17 -1.0 3,398,913 + PS417_15525 0.17 -0.1 3,398,913 + PS417_15525 0.17 -1.3 3,398,913 + PS417_15525 0.17 +0.1 3,398,913 + PS417_15525 0.17 -0.5 3,399,039 + PS417_15525 0.31 -2.0 3,399,040 - PS417_15525 0.32 -0.8 3,399,040 - PS417_15525 0.32 -0.4 3,399,156 + PS417_15525 0.45 +0.3 3,399,156 + PS417_15525 0.45 -0.4 3,399,156 + PS417_15525 0.45 -0.8 3,399,157 - PS417_15525 0.45 -0.9 3,399,157 - PS417_15525 0.45 -2.3 3,399,157 - PS417_15525 0.45 -0.9 3,399,646 + +0.3 3,399,825 + PS417_15530 0.32 +0.7 3,399,826 - PS417_15530 0.32 +0.0 3,399,826 - PS417_15530 0.32 -0.2 3,399,826 - PS417_15530 0.32 -0.2 3,399,928 + PS417_15530 0.48 +0.2 3,399,929 - PS417_15530 0.49 +0.5 3,400,053 + PS417_15530 0.68 +0.1 3,400,065 - PS417_15530 0.70 +0.7 3,400,076 + PS417_15530 0.72 +0.9 3,400,077 - PS417_15530 0.72 -0.5 3,400,077 - PS417_15530 0.72 -0.5 3,400,286 + +0.4 3,400,287 - +1.3 3,400,287 - -0.1 3,400,287 - -0.4 3,400,287 - +0.6 3,400,290 + +0.5 3,400,316 + -0.2 3,400,317 - -0.3
Or see this region's nucleotide sequence