Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09590 and PS417_09595 overlap by 1 nucleotides PS417_09595 and PS417_09600 are separated by 2 nucleotides
PS417_09590: PS417_09590 - membrane protein, at 2,076,269 to 2,077,678
_09590
PS417_09595: PS417_09595 - membrane protein, at 2,077,678 to 2,079,105
_09595
PS417_09600: PS417_09600 - glycosyl transferase family 1, at 2,079,108 to 2,080,211
_09600
Position (kb)
2077
2078
2079
2080 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2077.002 kb on - strand, within PS417_09590 at 2077.182 kb on + strand, within PS417_09590 at 2077.183 kb on - strand, within PS417_09590 at 2077.208 kb on + strand, within PS417_09590 at 2077.261 kb on + strand, within PS417_09590 at 2077.261 kb on + strand, within PS417_09590 at 2077.262 kb on - strand, within PS417_09590 at 2077.483 kb on - strand, within PS417_09590 at 2077.715 kb on - strand at 2077.826 kb on + strand, within PS417_09595 at 2077.827 kb on - strand, within PS417_09595 at 2077.827 kb on - strand, within PS417_09595 at 2077.994 kb on + strand, within PS417_09595 at 2077.994 kb on + strand, within PS417_09595 at 2077.995 kb on - strand, within PS417_09595 at 2077.995 kb on - strand, within PS417_09595 at 2078.000 kb on + strand, within PS417_09595 at 2078.000 kb on + strand, within PS417_09595 at 2078.001 kb on - strand at 2078.187 kb on - strand, within PS417_09595 at 2078.244 kb on - strand, within PS417_09595 at 2078.884 kb on - strand, within PS417_09595 at 2078.983 kb on + strand at 2078.983 kb on + strand at 2078.984 kb on - strand at 2079.376 kb on + strand, within PS417_09600 at 2079.377 kb on - strand, within PS417_09600 at 2079.809 kb on - strand at 2079.809 kb on - strand, within PS417_09600 at 2079.940 kb on + strand, within PS417_09600 at 2079.940 kb on + strand, within PS417_09600 at 2079.941 kb on - strand, within PS417_09600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,077,002 - PS417_09590 0.52 -1.1 2,077,182 + PS417_09590 0.65 -2.1 2,077,183 - PS417_09590 0.65 -1.6 2,077,208 + PS417_09590 0.67 -2.5 2,077,261 + PS417_09590 0.70 -1.8 2,077,261 + PS417_09590 0.70 -0.9 2,077,262 - PS417_09590 0.70 +0.8 2,077,483 - PS417_09590 0.86 -0.6 2,077,715 - +0.2 2,077,826 + PS417_09595 0.10 -1.1 2,077,827 - PS417_09595 0.10 -0.5 2,077,827 - PS417_09595 0.10 -2.1 2,077,994 + PS417_09595 0.22 -2.3 2,077,994 + PS417_09595 0.22 -1.2 2,077,995 - PS417_09595 0.22 -3.3 2,077,995 - PS417_09595 0.22 -0.8 2,078,000 + PS417_09595 0.23 -1.1 2,078,000 + PS417_09595 0.23 -1.6 2,078,001 - -0.6 2,078,187 - PS417_09595 0.36 -1.0 2,078,244 - PS417_09595 0.40 -1.9 2,078,884 - PS417_09595 0.84 -1.2 2,078,983 + +0.1 2,078,983 + -1.2 2,078,984 - -1.3 2,079,376 + PS417_09600 0.24 -3.0 2,079,377 - PS417_09600 0.24 -2.7 2,079,809 - +0.6 2,079,809 - PS417_09600 0.63 -2.1 2,079,940 + PS417_09600 0.75 -1.5 2,079,940 + PS417_09600 0.75 -1.4 2,079,941 - PS417_09600 0.75 -2.4
Or see this region's nucleotide sequence