Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02760 and PS417_02765 are separated by 71 nucleotides PS417_02765 and PS417_02770 are separated by 193 nucleotides PS417_02770 and PS417_02775 are separated by 135 nucleotides
PS417_02760: PS417_02760 - AraC family transcriptional regulator, at 628,243 to 629,031
_02760
PS417_02765: PS417_02765 - phosphate starvation-inducible protein PsiF, at 629,103 to 629,405
_02765
PS417_02770: PS417_02770 - membrane protein, at 629,599 to 630,612
_02770
PS417_02775: PS417_02775 - heat-shock protein, at 630,748 to 632,016
_02775
Position (kb) 629
630
631 Strain fitness (log2 ratio) -2
-1
0
1 at 629.037 kb on + strand at 629.038 kb on - strand at 629.051 kb on + strand at 629.051 kb on + strand at 629.051 kb on + strand at 629.052 kb on - strand at 629.183 kb on + strand, within PS417_02765 at 629.599 kb on - strand at 629.599 kb on - strand at 629.627 kb on + strand at 629.641 kb on + strand at 629.641 kb on + strand at 629.641 kb on + strand at 629.641 kb on + strand at 629.641 kb on + strand at 629.641 kb on + strand at 629.641 kb on + strand at 629.642 kb on - strand at 629.642 kb on - strand at 629.642 kb on - strand at 629.822 kb on + strand, within PS417_02770 at 629.823 kb on - strand, within PS417_02770 at 630.445 kb on + strand, within PS417_02770 at 630.807 kb on + strand at 631.268 kb on + strand, within PS417_02775 at 631.268 kb on + strand, within PS417_02775 at 631.269 kb on - strand, within PS417_02775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 629,037 + +1.4 629,038 - -1.5 629,051 + -0.0 629,051 + -1.9 629,051 + +0.5 629,052 - -0.3 629,183 + PS417_02765 0.26 +0.5 629,599 - +0.2 629,599 - -0.2 629,627 + +0.6 629,641 + +0.3 629,641 + -2.5 629,641 + +0.1 629,641 + -1.0 629,641 + +0.1 629,641 + +0.8 629,641 + -0.1 629,642 - -0.4 629,642 - -0.4 629,642 - +0.1 629,822 + PS417_02770 0.22 -0.3 629,823 - PS417_02770 0.22 -0.8 630,445 + PS417_02770 0.83 -1.2 630,807 + +0.3 631,268 + PS417_02775 0.41 -2.0 631,268 + PS417_02775 0.41 +0.1 631,269 - PS417_02775 0.41 +1.4
Or see this region's nucleotide sequence