Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18910 and PS417_18915 overlap by 4 nucleotides PS417_18915 and PS417_18920 are separated by 9 nucleotides PS417_18920 and PS417_18925 are separated by 54 nucleotides
PS417_18910: PS417_18910 - acetylornithine deacetylase, at 4,095,905 to 4,097,062
_18910
PS417_18915: PS417_18915 - hypothetical protein, at 4,097,059 to 4,097,733
_18915
PS417_18920: PS417_18920 - hypothetical protein, at 4,097,743 to 4,098,474
_18920
PS417_18925: PS417_18925 - flagellar motor switch protein FliY, at 4,098,529 to 4,099,359
_18925
Position (kb)
4097
4098 Strain fitness (log2 ratio)
-2
-1
0
1 at 4096.494 kb on + strand, within PS417_18910 at 4096.494 kb on + strand, within PS417_18910 at 4096.671 kb on + strand, within PS417_18910 at 4096.750 kb on - strand, within PS417_18910 at 4096.750 kb on - strand, within PS417_18910 at 4096.779 kb on + strand, within PS417_18910 at 4096.936 kb on - strand, within PS417_18910 at 4096.971 kb on + strand at 4096.972 kb on - strand at 4097.124 kb on - strand at 4097.385 kb on + strand, within PS417_18915 at 4097.386 kb on - strand, within PS417_18915 at 4097.821 kb on - strand, within PS417_18920 at 4097.821 kb on - strand, within PS417_18920 at 4098.127 kb on - strand, within PS417_18920 at 4098.310 kb on - strand, within PS417_18920 at 4098.530 kb on + strand at 4098.531 kb on - strand at 4098.581 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 4,096,494 + PS417_18910 0.51 +0.1 4,096,494 + PS417_18910 0.51 -0.3 4,096,671 + PS417_18910 0.66 -1.9 4,096,750 - PS417_18910 0.73 +0.5 4,096,750 - PS417_18910 0.73 -0.1 4,096,779 + PS417_18910 0.75 +1.0 4,096,936 - PS417_18910 0.89 -0.3 4,096,971 + -1.0 4,096,972 - +0.1 4,097,124 - +0.5 4,097,385 + PS417_18915 0.48 -0.8 4,097,386 - PS417_18915 0.48 -0.5 4,097,821 - PS417_18920 0.11 -1.1 4,097,821 - PS417_18920 0.11 -0.0 4,098,127 - PS417_18920 0.52 -0.2 4,098,310 - PS417_18920 0.77 -0.1 4,098,530 + +0.5 4,098,531 - +1.5 4,098,581 + -1.6
Or see this region's nucleotide sequence