Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00175 and PS417_00180 are separated by 136 nucleotides PS417_00180 and PS417_00185 overlap by 1 nucleotides PS417_00185 and PS417_00190 are separated by 110 nucleotides
PS417_00175: PS417_00175 - CigR, at 37,312 to 37,752
_00175
PS417_00180: PS417_00180 - tryptophan synthase subunit alpha, at 37,889 to 38,698
_00180
PS417_00185: PS417_00185 - tryptophan synthase subunit beta, at 38,698 to 39,939
_00185
PS417_00190: PS417_00190 - LysR family transcriptional regulator, at 40,050 to 40,946
_00190
Position (kb) 38
39
40 Strain fitness (log2 ratio) -1
0
1 at 37.826 kb on - strand at 37.826 kb on - strand at 37.894 kb on + strand at 37.894 kb on + strand at 37.894 kb on + strand at 37.895 kb on - strand at 38.175 kb on + strand, within PS417_00180 at 38.294 kb on - strand, within PS417_00180 at 38.395 kb on - strand, within PS417_00180 at 38.729 kb on - strand at 38.729 kb on - strand at 38.940 kb on - strand, within PS417_00185 at 39.354 kb on - strand, within PS417_00185 at 39.513 kb on - strand, within PS417_00185 at 39.513 kb on - strand, within PS417_00185 at 39.513 kb on - strand, within PS417_00185 at 39.646 kb on - strand, within PS417_00185 at 39.917 kb on - strand at 39.917 kb on - strand at 40.525 kb on - strand, within PS417_00190 at 40.728 kb on + strand at 40.731 kb on + strand, within PS417_00190 at 40.731 kb on + strand, within PS417_00190 at 40.732 kb on - strand at 40.927 kb on + strand at 40.928 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 37,826 - -0.6 37,826 - -0.5 37,894 + -0.7 37,894 + -0.3 37,894 + -0.2 37,895 - +1.3 38,175 + PS417_00180 0.35 +0.5 38,294 - PS417_00180 0.50 +0.5 38,395 - PS417_00180 0.62 -1.2 38,729 - +0.1 38,729 - +0.2 38,940 - PS417_00185 0.19 +0.4 39,354 - PS417_00185 0.53 -1.5 39,513 - PS417_00185 0.66 -0.8 39,513 - PS417_00185 0.66 -1.5 39,513 - PS417_00185 0.66 -1.0 39,646 - PS417_00185 0.76 +0.4 39,917 - -1.7 39,917 - -0.7 40,525 - PS417_00190 0.53 -0.5 40,728 + -0.5 40,731 + PS417_00190 0.76 +0.1 40,731 + PS417_00190 0.76 -0.1 40,732 - -1.2 40,927 + +0.0 40,928 - +0.1
Or see this region's nucleotide sequence